PPP2R2A Antibody - #DF6908

(3)  
製品: PPP2R2A Antibody
カタログ: DF6908
タンパク質の説明: Rabbit polyclonal antibody to PPP2R2A
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 52kDa; 52kD(Calculated).
ユニプロット: P63151
RRID: AB_2838867

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
PPP2R2A Antibody detects endogenous levels of total PPP2R2A.
RRID:
AB_2838867
引用形式: Affinity Biosciences Cat# DF6908, RRID:AB_2838867.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

2ABA_HUMAN; Alpha isoform of regulatory subunit B55 protein phosphatase 2; B55A; B55ALPHA; calcineurin; PP2A subunit B B alpha isoform; PP2A subunit B B55 alpha isoform; PP2A subunit B isoform alpha; PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B PR55 alpha isoform; PP2A subunit B R2 alpha isoform; PPP2R2A; PR52A; PR55A; Protein phosphatase 2 (formerly 2A) regulatory subunit B (PR 52) alpha isoform; Protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform; Protein phosphatase 2 regulatory subunit B alpha; Protein phosphatase 2 regulatory subunit B alpha isoform; Protein phosphatase 2 regulatory subunit Balpha; Serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; Testicular tissue protein Li 156;

免疫原

免疫原:
Uniprot:

P63151(2ABA_HUMAN) >>Visit HPA database.

遺伝子(ID):
PPP2R2A(5520)
発現特異性:
P63151 2ABA_HUMAN:

Expressed in all tissues examined.

タンパク質の説明:
Protein phosphatase 2A subunit B isoform R2-α (PPP2R2A) is the component of regulatory subunit B of serine/threonine protein phosphatase 2A (PP2A). PPP2R2A belongs to the R2/B55/PR55 family of regulatory subunit B (1). Evidence suggests that subunit PPP2R2A enhances TGF-β, Activin, and Nodal signaling pathways (7) and may play an important role in cell cycle regulation (2-4). PPP2R2A/PP2A, B55-α/PR55-α isoform interacts with the type I TGF-β receptors via unique WD-40 repeats (5) that contribute to the anti-proliferative function of TGF-β receptors (6).
タンパク質配列:
MAGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQENKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P63151 基板として

Site PTM Type Enzyme
A2 Acetylation
K62 Acetylation
K62 Ubiquitination
R68 Methylation
K88 Acetylation
K88 Ubiquitination
K95 Ubiquitination
K98 Acetylation
K105 Ubiquitination
K117 Ubiquitination
K123 Ubiquitination
K129 Ubiquitination
K137 Ubiquitination
S167 Phosphorylation
S266 Phosphorylation
K267 Ubiquitination
R278 Methylation
K292 Ubiquitination
K332 Ubiquitination
S335 Phosphorylation
S355 Phosphorylation
T359 Phosphorylation
T378 Phosphorylation
K387 Ubiquitination
S400 Phosphorylation Q13315 (ATM)
K402 Acetylation
K404 Ubiquitination
K405 Ubiquitination
S409 Phosphorylation
K417 Ubiquitination
K418 Ubiquitination
Y440 Phosphorylation

研究背景

機能:

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

組織特異性:

Expressed in all tissues examined.

サブユニット構造:

Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity). PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules (By similarity). Interacts with TP53. Interacts with IER5. Interacts with MFHAS1; the interaction is direct. Interacts with FAM122A. Interacts with CRTC3.

タンパク質ファミリー:

Belongs to the phosphatase 2A regulatory subunit B family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

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