TTK Antibody - #DF6969
(2)
| 製品: | TTK Antibody |
| カタログ: | DF6969 |
| タンパク質の説明: | Rabbit polyclonal antibody to TTK |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 97kDa; 97kD(Calculated). |
| ユニプロット: | P33981 |
| RRID: | AB_2838925 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
クローナリティ:
Polyclonal
特異性:
TTK Antibody detects endogenous levels of total TTK.
RRID:
AB_2838925
引用形式: Affinity Biosciences Cat# DF6969, RRID:AB_2838925.
引用形式: Affinity Biosciences Cat# DF6969, RRID:AB_2838925.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ECSTY kinase; ESK; FLJ38280; hMPS1; Monopolar Spindle 1 Like 1; MPH1; Mps 1; MPS1L1; Phosphotyrosine Picked Threonine Kinase; Phosphotyrosine picked threonine-protein kinase; PYT; RP3-357D13.2; ttk; TTK Protein Kinase; TTK_HUMAN;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質の説明:
This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
タンパク質配列:
- P33981 TTK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
75
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P33981 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | P33981 (TTK) | Uniprot |
| S15 | Phosphorylation | P33981 (TTK) | Uniprot |
| K19 | Methylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| K28 | Sumoylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| T33 | Phosphorylation | P33981 (TTK) | Uniprot |
| S37 | Phosphorylation | P33981 (TTK) | Uniprot |
| T46 | Phosphorylation | Uniprot | |
| K71 | Sumoylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S80 | Phosphorylation | P33981 (TTK) | Uniprot |
| K86 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S281 | Phosphorylation | P15056 (BRAF) | Uniprot |
| K287 | Sumoylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| T288 | Phosphorylation | O96017 (CHEK2) , P33981 (TTK) | Uniprot |
| S291 | Phosphorylation | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| T351 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| T360 | Phosphorylation | P33981 (TTK) | Uniprot |
| S362 | Phosphorylation | P33981 (TTK) | Uniprot |
| S363 | Phosphorylation | P33981 (TTK) | Uniprot |
| K367 | Sumoylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| Y374 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| K385 | Methylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S436 | Phosphorylation | P15056 (BRAF) , P33981 (TTK) | Uniprot |
| S440 | Phosphorylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| Y462 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| R467 | Methylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| K472 | Sumoylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| T564 | Phosphorylation | P33981 (TTK) | Uniprot |
| K577 | Ubiquitination | Uniprot | |
| S582 | Phosphorylation | P33981 (TTK) | Uniprot |
| K584 | Ubiquitination | Uniprot | |
| T594 | Phosphorylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| T675 | Phosphorylation | P33981 (TTK) | Uniprot |
| T676 | Phosphorylation | P33981 (TTK) | Uniprot |
| S677 | Phosphorylation | P33981 (TTK) | Uniprot |
| K680 | Ubiquitination | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| T686 | Phosphorylation | P33981 (TTK) | Uniprot |
| K696 | Ubiquitination | Uniprot | |
| K708 | Methylation | Uniprot | |
| S742 | Phosphorylation | P33981 (TTK) | Uniprot |
| K762 | Ubiquitination | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| T795 | Phosphorylation | P33981 (TTK) | Uniprot |
| K803 | Ubiquitination | Uniprot | |
| T805 | Phosphorylation | P33981 (TTK) | Uniprot |
| T806 | Phosphorylation | P33981 (TTK) | Uniprot |
| K810 | Ubiquitination | Uniprot | |
| Y811 | Phosphorylation | P33981 (TTK) | Uniprot |
| S821 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) , P33981 (TTK) | Uniprot |
| S824 | Phosphorylation | P33981 (TTK) | Uniprot |
| K827 | Ubiquitination | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| Y833 | Phosphorylation | Uniprot | |
| Y836 | Phosphorylation | Uniprot | |
| S837 | Phosphorylation | Uniprot | |
| S845 | Phosphorylation | P33981 (TTK) | Uniprot |
| S847 | Phosphorylation | P33981 (TTK) | Uniprot |
| K848 | Ubiquitination | Uniprot | |
| T849 | Phosphorylation | P33981 (TTK) | Uniprot |
| K852 | Acetylation | Uniprot | |
| K853 | Acetylation | Uniprot |
PTMs - P33981 酵素として
| Substrate | Site | Source |
|---|---|---|
| O60566 (BUB1B) | S435 | Uniprot |
| O60566 (BUB1B) | S543 | Uniprot |
| O60566 (BUB1B) | S670 | Uniprot |
| O60566 (BUB1B) | S1043 | Uniprot |
| O96017 (CHEK2) | T68 | Uniprot |
| P00519 (ABL1) | T735 | Uniprot |
| P04637 (TP53) | T18 | Uniprot |
| P07900 (HSP90AA1) | T115 | Uniprot |
| P33981 (TTK) | T12 | Uniprot |
| P33981 (TTK) | S15 | Uniprot |
| P33981 (TTK) | T33 | Uniprot |
| P33981 (TTK) | S37 | Uniprot |
| P33981 (TTK) | S80 | Uniprot |
| P33981 (TTK) | T288 | Uniprot |
| P33981 (TTK) | T360 | Uniprot |
| P33981 (TTK) | S362 | Uniprot |
| P33981 (TTK) | S363 | Uniprot |
| P33981 (TTK) | S436 | Uniprot |
| P33981 (TTK) | T564 | Uniprot |
| P33981 (TTK) | S582 | Uniprot |
| P33981 (TTK) | T675 | Uniprot |
| P33981 (TTK) | T676 | Uniprot |
| P33981 (TTK) | S677 | Uniprot |
| P33981 (TTK) | T686 | Uniprot |
| P33981 (TTK) | S742 | Uniprot |
| P33981 (TTK) | T795 | Uniprot |
| P33981 (TTK) | T805 | Uniprot |
| P33981 (TTK) | T806 | Uniprot |
| P33981 (TTK) | Y811 | Uniprot |
| P33981 (TTK) | S821 | Uniprot |
| P33981 (TTK) | S824 | Uniprot |
| P33981 (TTK) | S845 | Uniprot |
| P33981 (TTK) | S847 | Uniprot |
| P33981 (TTK) | T849 | Uniprot |
| P38646 (HSPA9) | T62 | Uniprot |
| P38646 (HSPA9) | S65 | Uniprot |
| P54132 (BLM) | S144 | Uniprot |
| P54132 (BLM) | S1290 | Uniprot |
| P54132 (BLM) | S1296 | Uniprot |
| Q53HL2 (CDCA8) | T88 | Uniprot |
| Q53HL2 (CDCA8) | T94 | Uniprot |
| Q53HL2 (CDCA8) | T169 | Uniprot |
| Q53HL2 (CDCA8) | T230 | Uniprot |
| Q53HL2 (CDCA8) | S238 | Uniprot |
| Q96E14 (RMI2) | S112 | Uniprot |
研究背景
機能:
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
組織特異性:
Present in rapidly proliferating cell lines.
サブユニット構造:
Interacts with TPR; the interactions occurs in a microtubule-independent manner.
タンパク質ファミリー:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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