CASK Antibody - #DF6970
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF6970, RRID:AB_2838926.
折りたたみ／展開
CAGH39; Caki; Calcium/calmodulin dependent serine protein kinase; Calcium/calmodulin dependent serine protein kinase (MAGUK family); Calcium/calmodulin dependent serine protein kinase membrane associated guanylate kinase; Calcium/calmodulin-dependent serine protein kinase; CAMGUK; CAMGUK protein; CAMGUK, drosophila, homolog of; casK; CMG; CSKP_HUMAN; DXPri1; DXRib1; FGS4; FLJ22219; FLJ31914; hCASK; LIN 2; Lin 2 homolog; LIN2; Lin2 homolog; MICPCH; MRXSNA; Pals3; Peripheral plasma membrane protein CASK; Protein lin-2 homolog; TNRC8; Trinucleotide repeat containing 8; Vertebtate LIN2 homolog;
免疫原
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
- O14936 CSKP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGLLAAERAVSQVLDSLEEIHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAKELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKNTDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFKIVPSYRTQSSSCERDSPSTSRQSPANGHSSTNNSVSDLPSTTQPKGRQIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQGKLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNAVFDQLDLVTYEEVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPDRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQDISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNEDESLQRLQKESDILQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14936 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K41 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | O14936 (CASK) | Uniprot |
| S155 | Phosphorylation | O14936 (CASK) | Uniprot |
| T182 | Phosphorylation | Uniprot | |
| Y282 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| K410 | Ubiquitination | Uniprot | |
| T460 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S570 | Phosphorylation | Uniprot | |
| Y571 | Phosphorylation | Uniprot | |
| T573 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| K665 | Ubiquitination | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| K703 | Methylation | Uniprot | |
| K703 | Ubiquitination | Uniprot | |
| K704 | Methylation | Uniprot | |
| Y708 | Phosphorylation | Uniprot | |
| Y712 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K738 | Methylation | Uniprot | |
| T741 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K756 | Ubiquitination | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| Y769 | Phosphorylation | Uniprot | |
| T775 | Phosphorylation | Uniprot | |
| Y788 | Phosphorylation | Uniprot | |
| Y789 | Phosphorylation | Uniprot | |
| S792 | Phosphorylation | Uniprot | |
| K825 | Ubiquitination | Uniprot | |
| K843 | Ubiquitination | Uniprot |
研究背景
Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
Nucleus. Cytoplasm. Cell membrane>Peripheral membrane protein.
Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
CASK and LIN7 form two mutually exclusive tripartite complexes with APBA1 or CASKIN1 (By similarity). Forms a heterotrimeric complex with DLG1 and LIN7B via their L27 domains (By similarity). Identified in a complex with ACTN4, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Part of a complex containing CASK, TBR1 and TSPYL2 (By similarity). Interacts with WHRN (By similarity). Interacts (via the PDZ, SH3 and guanylate kinase-like domains) with NRXN1 (via C-terminus) (By similarity). Interacts with CASKIN1, APBA1, LIN7(A/B/C) and L27 domain of DLG1 and isoform 2 of DLG4 (By similarity). Interacts with FCHSD2 (By similarity). Interacts with KIRREL3. Interacts with TBR1 (By similarity). Interacts with TSPYL2 (By similarity).
The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7.
The protein kinase domain mediates the interaction with FCHSD2.
In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Belongs to the MAGUK family.
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