EEF1D Antibody - #DF6974

(1)  
製品: EEF1D Antibody
カタログ: DF6974
タンパク質の説明: Rabbit polyclonal antibody to EEF1D
アプリケーション: WB IHC
反応性: Human, Mouse, Rat, Monkey
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 31kDa,37kDa; 31kD(Calculated).
ユニプロット: P29692
RRID: AB_2838930

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
EEF1D Antibody detects endogenous levels of total EEF1D.
RRID:
AB_2838930
引用形式: Affinity Biosciences Cat# DF6974, RRID:AB_2838930.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); Eukaryotic translation elongation factor 1 delta; FP1047; Guanine nucleotide exchange protein;

免疫原

免疫原:

A synthesized peptide derived from human EEF1D, corresponding to a region within N-terminal amino acids.

Uniprot:

P29692(EF1D_HUMAN) >>Visit HPA database.

遺伝子(ID):
EEF1D(1936)
発現特異性:
P29692 EF1D_HUMAN:

Isoform 2 is specifically expressed in brain, cerebellum and testis.

タンパク質の説明:
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
タンパク質配列:
MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.

Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 2 is specifically expressed in brain, cerebellum and testis.

タンパク質ファミリー:

Belongs to the EF-1-beta/EF-1-delta family.

研究領域

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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