FLIP Antibody - #DF7010

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製品: FLIP Antibody
カタログ: DF7010
タンパク質の説明: Rabbit polyclonal antibody to FLIP
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 52kDa; 55kD(Calculated).
ユニプロット: O15519
RRID: AB_2838966

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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
FLIP Antibody detects endogenous levels of total FLIP.
RRID:
AB_2838966
引用形式: Affinity Biosciences Cat# DF7010, RRID:AB_2838966.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8AP1; Caspase homolog; Caspase like apoptosis regulatory protein; Caspase related inducer of apoptosis; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; Casper; Cellular FLICE-like inhibitory protein; CFLA; Cflar; CFLAR_HUMAN; CLARP; FADD like anti apoptotic molecule; FADD-like antiapoptotic molecule 1; FLAME; FLAME-1; FLAME1; FLIP; I FLICE; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; OTTHUMP00000163715; OTTHUMP00000206475; OTTHUMP00000206476; OTTHUMP00000206478; OTTHUMP00000206479; OTTHUMP00000206480; OTTHUMP00000206482; OTTHUMP00000207360; Usurpin; Usurpin beta;

免疫原

免疫原:
Uniprot:

O15519(CFLAR_HUMAN) >>Visit HPA database.

遺伝子(ID):
CFLAR(8837)
発現特異性:
O15519 CFLAR_HUMAN:

Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

タンパク質の説明:
Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis that has various names, such as c-FLIP (1), Casper (2), CLARP (3), FLAME (4), I-FLICE (5), MRIT (6), CASH (7), and Usurpin (8). FLIP is expressed as two alternative splice isoforms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) like those found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE), and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both FLIP isoforms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some research studies have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease, resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkin's lymphoma and ovarian and colon carcinomas (9).
タンパク質配列:
MSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVLMAEIGEDLDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQSVQGAGTSYRNVLQAAIQKSLKDPSNNFRLHNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYKMKSKPLGICLIIDCIGNETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMPEHRDYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAGKPKMFFIQNYVVSEGQLEDSSLLEVDGPAMKNVEFKAQKRGLCTVHREADFFWSLCTADMSLLEQSHSSPSLYLQCLSQKLRQERKRPLLDLHIELNGYMYDWNSRVSAKEKYYVWLQHTLRKKLILSYT

PTMs - O15519 基板として

Site PTM Type Enzyme
K49 Ubiquitination
K77 Ubiquitination
R122 Methylation
K154 Ubiquitination
K157 Ubiquitination
T166 Phosphorylation
K167 Ubiquitination
Y171 Phosphorylation
K172 Ubiquitination
Y182 Phosphorylation
K192 Ubiquitination
S193 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB)
K195 Ubiquitination
K214 Ubiquitination
K225 Ubiquitination
K226 Ubiquitination
S227 Phosphorylation
S231 Phosphorylation
C254 S-Nitrosylation
C259 S-Nitrosylation
S273 Phosphorylation P31749 (AKT1)
S314 Phosphorylation
K386 Ubiquitination

研究背景

機能:

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

PTMs:

Proteolytically processed by CASP8 generating subunit p43 and p12.

組織特異性:

Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

サブユニット構造:

TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and CASP8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with FADD, CASP8, CASP3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with RIPK1 (By similarity).

(Microbial infection) Interacts with HBV protein X.

タンパク質ファミリー:

The caspase domain lacks the active site residues involved in catalysis.

Belongs to the peptidase C14A family.

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

参考文献

1). Nuclear respiratory factor 1 protects H9C2 cells against hypoxia‐induced apoptosis via the death receptor pathway and mitochondrial pathway. CELL BIOLOGY INTERNATIONAL, 2021 (PubMed: 33913583) [IF=3.9]

Application: WB    Species: Mouse    Sample: H9C2 cells

Figure 5. Effects of nuclear respiratory factor 1 (NRF-1) on the expression of death reporter pathway-associated genes and proteins in hypoxia-induced H9C2 cells A. The mRNA expression of NRF-1, Fas, FasL, FADD, Cflar, Caspase-8 and Bid were detected by RT-qPCR. β-actin was used as the internal control; B. The protein expression of NRF-1, Fas, FasL, FADD, Cflar, Caspase-8 and Bid was detected by western blot analysis. β-actin was used as the internal control. Band intensity ratios for each group are presented as the mean ± standard deviation (n=3). *p < 0.05 and **p < 0.01; NS, not significant.

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