DIABLO Antibody - #DF7017
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7017, RRID:AB_2838973.
折りたたみ／展開
0610041G12Rik; DBLOH_HUMAN; DBOH; DFNA64; diablo; Diablo homolog (Drosophila); Diablo homolog; Diablo homolog mitochondrial; Diablo IAP binding mitochondrial protein; Diablo like protein; DIABLO S; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second mitochondria derived activator of caspase; Second mitochondria-derived activator of caspase; SMAC 3; Smac; Smac protein; SMAC3;
免疫原
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.
- Q9NR28 DBLOH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALKSWLSRSVTSFFRYRQCLCVPVVANFKKRCFSELIRPWHKTVTIGFGVTLCAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED
PTMs - Q9NR28 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| S9 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| S11 | Phosphorylation | Uniprot | |
| T13 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K123 | Ubiquitination | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| T220 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot |
研究背景
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Ubiquitinated by BIRC7/livin.
Mitochondrion.
Note: Released into the cytosol when cells undergo apoptosis.
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.
Homodimer. Interacts with BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin.
The mature N-terminus mediates interaction with XIAP/BIRC4.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
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