SDHA Antibody - #DF7043
(1)
(5)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7043, RRID:AB_2838999.
折りたたみ／展開
CMD1GG; DHSA_HUMAN; Flavoprotein subunit of complex II; Fp; PGL5; SDH 2; SDH1; SDH2; SDHA; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Succinate dehydrogenase complex flavoprotein subunit A; Succinate dehydrogenase complex flavoprotein subunit; Succinate dehydrogenase complex flavoprotein subunit precursor; Succinate dehydrogenase complex subunit A; Succinate dehydrogenase complex subunit A flavoprotein (Fp); Succinate dehydrogenase complex subunit A flavoprotein;
免疫原
- P31040 SDHA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGVRGLSRLLSARRLALAKAWPTVLQTGTRGFHFTVDGNKRASAKVSDSISAQYPVVDHEFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKLFPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIHYMTEQAPAAVVELENYGMPFSRTEDGKIYQRAFGGQSLKFGKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIEDGSIHRIRAKNTVVATGGYGRTYFSCTSAHTSTGDGTAMITRAGLPCQDLEFVQFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P31040 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T24 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| Y215 | Phosphorylation | P12931 (SRC) | Uniprot |
| S243 | Phosphorylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K335 | Acetylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K361 | Acetylation | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| Y436 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K498 | Acetylation | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| R500 | Methylation | Uniprot | |
| R507 | Methylation | Uniprot | |
| R512 | Methylation | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| K541 | Acetylation | Uniprot | |
| K541 | Ubiquitination | Uniprot | |
| Y543 | Phosphorylation | P09769 (FGR) | Uniprot |
| K547 | Acetylation | Uniprot | |
| K547 | Methylation | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| R585 | Methylation | Uniprot | |
| R589 | Methylation | Uniprot | |
| R594 | Methylation | Uniprot | |
| K598 | Acetylation | Uniprot | |
| Y604 | Phosphorylation | P09769 (FGR) | Uniprot |
| Y606 | Phosphorylation | Uniprot | |
| K608 | Acetylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| K624 | Acetylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| Y629 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| R662 | Methylation | Uniprot |
研究背景
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.
Acetylated. Deacetylated by SIRT3.
Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.
Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (By similarity). Interacts with SDHAF2/SDH5; interaction is required for FAD attachment. Interacts with TRAP1.
Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
研究領域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
参考文献
Application: WB Species: human Sample: ACHN cells
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