Gelsolin Antibody - #DF7117
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7117, RRID:AB_2839071.
折りたたみ／展開
Actin depolymerizing factor; Actin-depolymerizing factor; ADF; AGEL; Brevin; DKFZp313L0718; GELS_HUMAN; Gelsolin; Gsn;
免疫原
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
- P06396 GELS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPHRPAPALLCALSLALCALSLPVRAATASRGASQAGAPQGRVPEARPNSMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWESFNNGDCFILDLGNNIHQWCGSNSNRYERLKATQVSKGIRDNERSGRARVHVSEEGTEPEAMLQVLGPKPALPAGTEDTAKEDAANRKLAKLYKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALKTASDFITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P06396 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S35 | O-Glycosylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | P12931 (SRC) | Uniprot |
| K239 | Ubiquitination | Uniprot | |
| K245 | Acetylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| T386 | Phosphorylation | Uniprot | |
| K390 | Acetylation | Uniprot | |
| R397 | Methylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| Y409 | Phosphorylation | P12931 (SRC) | Uniprot |
| T431 | O-Glycosylation | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| Y465 | Phosphorylation | P12931 (SRC) | Uniprot |
| S473 | Phosphorylation | Uniprot | |
| Y478 | Phosphorylation | Uniprot | |
| Y480 | Phosphorylation | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| Y603 | Phosphorylation | P12931 (SRC) | Uniprot |
| S611 | Phosphorylation | Uniprot | |
| S636 | Phosphorylation | Uniprot | |
| K648 | Acetylation | Uniprot | |
| Y651 | Phosphorylation | P12931 (SRC) | Uniprot |
| S654 | Phosphorylation | Uniprot | |
| K728 | Acetylation | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| T742 | Phosphorylation | Uniprot |
研究背景
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Cytoplasm>Cytoskeleton.
Secreted.
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Binds to actin and to fibronectin. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X. Interacts with the inactive form of EIF2AK2/PKR (By similarity).
Belongs to the villin/gelsolin family.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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