ABCC1 Antibody - #DF7148

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製品: ABCC1 Antibody
カタログ: DF7148
タンパク質の説明: Rabbit polyclonal antibody to ABCC1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Zebrafish
分子量: 173kDa; 172kD(Calculated).
ユニプロット: P33527
RRID: AB_2839101

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(92%)
クローナリティ:
Polyclonal
特異性:
ABCC1 Antibody detects endogenous levels of total ABCC1.
RRID:
AB_2839101
引用形式: Affinity Biosciences Cat# DF7148, RRID:AB_2839101.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ABC 29; ABC29; ABCC 1; ABCC; Abcc1; ATP binding cassette sub family C (CFTR/MRP) member 1; ATP binding cassette sub-family C member 1; ATP binding cassette subfamily C member 1; ATP binding cassette transporter variant ABCC1delta ex13; ATP binding cassette transporter variant ABCC1delta ex13&14; ATP binding cassette transporter variant ABCC1delta ex25; ATP binding cassette transporter variant ABCC1delta ex25&26; ATP binding cassette, sub-family C (CFTR/MRP), member 1; ATP-binding cassette sub-family C member 1; DKFZp686N04233; DKFZp781G125; GS X; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP 1; MRP; MRP1; MRP1_HUMAN; Multidrug resistance associated protein 1; Multidrug resistance protein; Multidrug resistance-associated protein 1; Multiple drug resistance associated protein; Multiple drug resistance protein 1;

免疫原

免疫原:
Uniprot:

P33527(MRP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
ABCC1(4363)
発現特異性:
P33527 MRP1_HUMAN:

Lung, testis and peripheral blood mononuclear cells.

タンパク質の説明:
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
タンパク質配列:
MALRGFCSADGSDPLWDWNVTWNTSNPDFTKCFQNTVLVWVPCFYLWACFPFYFLYLSRHDRGYIQMTPLNKTKTALGFLLWIVCWADLFYSFWERSRGIFLAPVFLVSPTLLGITMLLATFLIQLERRKGVQSSGIMLTFWLVALVCALAILRSKIMTALKEDAQVDLFRDITFYVYFSLLLIQLVLSCFSDRSPLFSETIHDPNPCPESSASFLSRITFWWITGLIVRGYRQPLEGSDLWSLNKEDTSEQVVPVLVKNWKKECAKTRKQPVKVVYSSKDPAQPKESSKVDANEEVEALIVKSPQKEWNPSLFKVLYKTFGPYFLMSFFFKAIHDLMMFSGPQILKLLIKFVNDTKAPDWQGYFYTVLLFVTACLQTLVLHQYFHICFVSGMRIKTAVIGAVYRKALVITNSARKSSTVGEIVNLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPVNAVMAMKTKTYQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAYLSAVGTFTWVCTPFLVALCTFAVYVTIDENNILDAQTAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRIFLSHEELEPDSIERRPVKDGGGTNSITVRNATFTWARSDPPTLNGITFSIPEGALVAVVGQVGCGKSSLLSALLAEMDKVEGHVAIKGSVAYVPQQAWIQNDSLRENILFGCQLEEPYYRSVIQACALLPDLEILPSGDRTEIGEKGVNLSGGQKQRVSLARAVYSNADIYLFDDPLSAVDAHVGKHIFENVIGPKGMLKNKTRILVTHSMSYLPQVDVIIVMSGGKISEMGSYQELLARDGAFAEFLRTYASTEQEQDAEENGVTGVSGPGKEAKQMENGMLVTDSAGKQLQRQLSSSSSYSGDISRHHNSTAELQKAEAKKEETWKLMEADKAQTGQVKLSVYWDYMKAIGLFISFLSIFLFMCNHVSALASNYWLSLWTDDPIVNGTQEHTKVRLSVYGALGISQGIAVFGYSMAVSIGGILASRCLHVDLLHSILRSPMSFFERTPSGNLVNRFSKELDTVDSMIPEVIKMFMGSLFNVIGACIVILLATPIAAIIIPPLGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIHQSDLKVDENQKAYYPSIVANRWLAVRLECVGNCIVLFAALFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVAVERLKEYSETEKEAPWQIQETAPPSSWPQVGRVEFRNYCLRYREDLDFVLRHINVTINGGEKVGIVGRTGAGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKDFVSALPDKLDHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFEDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMAKDAGLV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
92
Bovine
75
Dog
67
Chicken
67
Xenopus
58
Pig
0
Horse
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33527 基板として

Site PTM Type Enzyme
N19 N-Glycosylation
N23 N-Glycosylation
K72 Ubiquitination
K246 Ubiquitination
T249 Phosphorylation P68400 (CSNK2A1)
K259 Ubiquitination
K274 Ubiquitination
Y277 Phosphorylation
S279 Phosphorylation
K280 Ubiquitination
S289 Phosphorylation
K290 Ubiquitination
K303 Ubiquitination
K307 Ubiquitination
K315 Ubiquitination
K396 Ubiquitination
Y404 Phosphorylation
K406 Ubiquitination
K416 Ubiquitination
K488 Ubiquitination
K496 Ubiquitination
S497 Phosphorylation
K498 Acetylation
K498 Ubiquitination
K503 Acetylation
K503 Ubiquitination
K513 Ubiquitination
K525 Ubiquitination
K527 Ubiquitination
K636 Ubiquitination
K764 Ubiquitination
K773 Ubiquitination
K804 Ubiquitination
K814 Ubiquitination
K818 Ubiquitination
S847 Phosphorylation
Y852 Phosphorylation
S871 Phosphorylation
T872 Phosphorylation
K891 Ubiquitination
K894 Ubiquitination
S905 Phosphorylation
K908 Ubiquitination
S915 Phosphorylation
S916 Phosphorylation
S917 Phosphorylation
S918 Phosphorylation
S919 Phosphorylation
Y920 Phosphorylation
S921 Phosphorylation
S925 Phosphorylation
S930 Phosphorylation
T931 Phosphorylation
K936 Ubiquitination
K946 Ubiquitination
K952 Ubiquitination
S961 Phosphorylation
N1006 N-Glycosylation
K1078 Ubiquitination
S1145 Phosphorylation
K1181 Ubiquitination
K1187 Ubiquitination
Y1243 Phosphorylation
K1265 Ubiquitination
K1272 Ubiquitination
K1322 Ubiquitination
K1333 Ubiquitination
K1360 Ubiquitination
K1369 Ubiquitination
K1502 Ubiquitination
Y1508 Phosphorylation
Y1522 Phosphorylation
S1523 Phosphorylation
K1526 Ubiquitination

研究背景

機能:

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation. Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Lung, testis and peripheral blood mononuclear cells.

タンパク質ファミリー:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

研究領域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

参考文献

1). 4′-OH as the Action Site of Lipids and MRP1 for Enhanced Transdermal Delivery of Flavonoids. ACS Applied Materials & Interfaces, 2023 (PubMed: 36913526) [IF=9.5]
2). miR-506 enhances the sensitivity of human colorectal cancer cells to oxaliplatin by suppressing MDR1/P-gp expression. CELL PROLIFERATION, 2017 (PubMed: 28217977) [IF=8.5]

Application: WB    Species: human    Sample:

FIGURE 3  miR-506 down-regulated MDR1/P-gp expression in HCT116-OxR. A, The mRNA level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant genes as demonstrated by qRT-PCR. B, The protein level of MDR1 was decreased after transfection with the miR-506 mimic of the relative chemoresistant proteins as demonstrated by Western blot. C, Expression of P-gp detected by immunofluorescence staining.HCT116-OxR-miR-506 cells showed low levels of fluorescent staining of P-gp, whereas maximal staining of P-gp was observed in HCT116-OxR cells, readily distinguished from background. Zoom: 200×. *P<.05)
3). miR‐485's anti‐drug resistant epilepsy effects by regulating SV2A/PSD‐95 and targeting ABCC1 and neuronal signaling‐transduction proteins in hippocampus of rats. Brain and Behavior, 2021 (PubMed: 34291586) [IF=3.1]

Application: WB    Species: Rat    Sample: hippocampus

FIGURE 5 The expressions of drug‐resistant‐related proteins (ABCC1) in hippocampus of DRE rats at 6, 7, and 9 weeks after SE. *p < .05 versus the control group; # p < .5 versus the epilepsy group

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