ABCC5 Antibody - #DF7149

(1)   (3)  
製品: ABCC5 Antibody
カタログ: DF7149
タンパク質の説明: Rabbit polyclonal antibody to ABCC5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 161kDa; 161kD(Calculated).
ユニプロット: O15440
RRID: AB_2839102

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
ABCC5 Antibody detects endogenous levels of total ABCC5.
RRID:
AB_2839102
引用形式: Affinity Biosciences Cat# DF7149, RRID:AB_2839102.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ABC 33; ABC33; ABCC 5; Abcc5; ATP binding cassette sub family C (CFTR/MRP) member 5; ATP binding cassette sub family C member 5; ATP-binding cassette sub-family C member 5; Canalicular multispecific organic anion transporter C; DKFZp686C1782; EST277145; MOAT C; MOAT-C; MOATC; MRP 5; MRP5_HUMAN; Multi specific organic anion tranporter C; Multi-specific organic anion transporter C; Multidrug resistance associated protein 5; Multidrug resistance-associated protein 5; pABC 11; pABC11; SMRP;

免疫原

免疫原:
Uniprot:

O15440(MRP5_HUMAN) >>Visit HPA database.

遺伝子(ID):
ABCC5(10057)
発現特異性:
O15440 MRP5_HUMAN:

All isoforms are equally expressed in retina.

タンパク質の説明:
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine.
タンパク質配列:
MKDIDIGKEYIIPSPGYRSVRERTSTSGTHRDREDSKFRRTRPLECQDALETAARAEGLSLDASMHSQLRILDEEHPKGKYHHGLSALKPIRTTSKHQHPVDNAGLFSCMTFSWLSSLARVAHKKGELSMEDVWSLSKHESSDVNCRRLERLWQEELNEVGPDAASLRRVVWIFCRTRLILSIVCLMITQLAGFSGPAFMVKHLLEYTQATESNLQYSLLLVLGLLLTEIVRSWSLALTWALNYRTGVRLRGAILTMAFKKILKLKNIKEKSLGELINICSNDGQRMFEAAAVGSLLAGGPVVAILGMIYNVIILGPTGFLGSAVFILFYPAMMFASRLTAYFRRKCVAATDERVQKMNEVLTYIKFIKMYAWVKAFSQSVQKIREEERRILEKAGYFQSITVGVAPIVVVIASVVTFSVHMTLGFDLTAAQAFTVVTVFNSMTFALKVTPFSVKSLSEASVAVDRFKSLFLMEEVHMIKNKPASPHIKIEMKNATLAWDSSHSSIQNSPKLTPKMKKDKRASRGKKEKVRQLQRTEHQAVLAEQKGHLLLDSDERPSPEEEEGKHIHLGHLRLQRTLHSIDLEIQEGKLVGICGSVGSGKTSLISAILGQMTLLEGSIAISGTFAYVAQQAWILNATLRDNILFGKEYDEERYNSVLNSCCLRPDLAILPSSDLTEIGERGANLSGGQRQRISLARALYSDRSIYILDDPLSALDAHVGNHIFNSAIRKHLKSKTVLFVTHQLQYLVDCDEVIFMKEGCITERGTHEELMNLNGDYATIFNNLLLGETPPVEINSKKETSGSQKKSQDKGPKTGSVKKEKAVKPEEGQLVQLEEKGQGSVPWSVYGVYIQAAGGPLAFLVIMALFMLNVGSTAFSTWWLSYWIKQGSGNTTVTRGNETSVSDSMKDNPHMQYYASIYALSMAVMLILKAIRGVVFVKGTLRASSRLHDELFRRILRSPMKFFDTTPTGRILNRFSKDMDEVDVRLPFQAEMFIQNVILVFFCVGMIAGVFPWFLVAVGPLVILFSVLHIVSRVLIRELKRLDNITQSPFLSHITSSIQGLATIHAYNKGQEFLHRYQELLDDNQAPFFLFTCAMRWLAVRLDLISIALITTTGLMIVLMHGQIPPAYAGLAISYAVQLTGLFQFTVRLASETEARFTSVERINHYIKTLSLEAPARIKNKAPSPDWPQEGEVTFENAEMRYRENLPLVLKKVSFTIKPKEKIGIVGRTGSGKSSLGMALFRLVELSGGCIKIDGVRISDIGLADLRSKLSIIPQEPVLFSGTVRSNLDPFNQYTEDQIWDALERTHMKECIAQLPLKLESEVMENGDNFSVGERQLLCIARALLRHCKILILDEATAAMDTETDLLIQETIREAFADCTMLTIAHRLHTVLGSDRIMVLAQGQVVEFDTPSVLLSNDSSRFYAMFAAAENKVAVKG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
56
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15440 基板として

Site PTM Type Enzyme
K8 Ubiquitination
Y10 Phosphorylation
S14 Phosphorylation
Y17 Phosphorylation
S19 Phosphorylation
S60 Phosphorylation
S64 Phosphorylation
S67 Phosphorylation
K78 Ubiquitination
K89 Ubiquitination
T351 Phosphorylation
K383 Ubiquitination
S485 Phosphorylation
K493 Ubiquitination
S501 Phosphorylation
S505 Phosphorylation
S509 Phosphorylation
T513 Phosphorylation
K546 Ubiquitination
S553 Phosphorylation
S558 Phosphorylation
K565 Ubiquitination
S686 Phosphorylation
K798 Acetylation
T800 Phosphorylation
K805 Acetylation
K806 Acetylation
S807 Phosphorylation
T814 Phosphorylation
K821 Ubiquitination
K824 Ubiquitination
K977 Ubiquitination
Y1166 Phosphorylation
Y1202 Phosphorylation
K1211 Ubiquitination
K1212 Ubiquitination
T1216 Phosphorylation
K1218 Ubiquitination
K1222 Ubiquitination
S1268 Phosphorylation
K1309 Ubiquitination
Y1423 Phosphorylation

研究背景

機能:

Acts as a multispecific organic anion pump which can transport nucleotide analogs.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

All isoforms are equally expressed in retina.

タンパク質ファミリー:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

研究領域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

参考文献

1). Brusatol Enhances the Chemotherapy Efficacy of Gemcitabine in Pancreatic Cancer via the Nrf2 Signalling Pathway. Oxidative Medicine and Cellular Longevity, 2018 (PubMed: 29849873)

Application: WB    Species: human    Sample: pancreatic cancer cells

Figure 2:| Brusatol inhibited the Nrf2 pathway and increase ROS accumulation in pancreatic cancer cells. (a) 0.5μM brusatol was utilized to treat the expression of Nrf2 in PATU-8988 cells for the indicated time points. (b) 0.5μM brusatol lowered the protein levels of Nrf2 and its downstreamgenesafter8-hourtreatments.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.