HCRTR2 Antibody - #DF7162

(1)   (6)  
製品: HCRTR2 Antibody
カタログ: DF7162
タンパク質の説明: Rabbit polyclonal antibody to HCRTR2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 51kDa; 51kD(Calculated).
ユニプロット: O43614
RRID: AB_2839114

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
HCRTR2 Antibody detects endogenous levels of total HCRTR2.
RRID:
AB_2839114
引用形式: Affinity Biosciences Cat# DF7162, RRID:AB_2839114.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Hcr tr2; Hcrt receptor 2; Hcrtr2; Hypocretin (orexin) receptor 2; Hypocretin receptor 2; Hypocretin receptor type 2; Orexin 2 receptor; Orexin A/Orexin B receptor; Orexin receptor 2; Orexin receptor type 2; Orexin type 2 receptor; Ox-2-R; Ox2-R; Ox2r; OX2R_HUMAN; Type 2 hypocretin receptor; Type 2 orexin receptor;

免疫原

免疫原:
Uniprot:

O43614(OX2R_HUMAN) >>Visit HPA database.

遺伝子(ID):
HCRTR2(3062)
タンパク質の説明:
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A.
タンパク質配列:
MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHPKEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNSIVIIWIVSCIIMIPQAIVMECSTVFPGLANKTTLFTVCDERWGGEIYPKMYHICFFLVTYMAPLCLMVLAYLQIFRKLWCRQIPGTSSVVQRKWKPLQPVSQPRGPGQPTKSRMSAVAAEIKQIRARRKTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREEFKAAFSCCCLGVHHRQEDRLTRGRTSTESRKSLTTQISNFDNISKLSEQVVLTSISTLPAANGAGPLQNW

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43614 基板として

Site PTM Type Enzyme
Y223 Phosphorylation
T231 Phosphorylation
Y243 Phosphorylation
S273 Phosphorylation
Y317 Phosphorylation
S321 Phosphorylation

研究背景

機能:

Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The N-terminal region is required for orexin signaling.

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

参考文献

1). Soporific effect of modified Suanzaoren Decoction on mice models of insomnia by regulating Orexin-A and HPA axis homeostasis. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34509822) [IF=7.5]

Application: WB    Species: Mice    Sample: hypothalamus tissue

Fig. 9. Effect of MSZRD on the level of Orexin-A and the expression of OX2R. (a) The level of Orexin-A in serum was measured by ELISA kits. (b) OX2R protein was quantified as IOD/Area by Imagine Pro. (c) The expression of OX2R in hypothalamus was detected by western blot and (d) Statistical analysis on OX2R. (e) The expression of OX2R protein in hypothalamus tissue was examined by immunohistochemical staining (400×，brown-yellow granules indicate positive expression). Data are expressed as the mean ± SD. # P < 0.05, ## P < 0.01 compare with control group; *P < 0.05 and **P < 0.01 compare with model group.

Application: IHC    Species: Mice    Sample: hypothalamus tissue

Fig. 9. Effect of MSZRD on the level of Orexin-A and the expression of OX2R. (a) The level of Orexin-A in serum was measured by ELISA kits. (b) OX2R protein was quantified as IOD/Area by Imagine Pro. (c) The expression of OX2R in hypothalamus was detected by western blot and (d) Statistical analysis on OX2R. (e) The expression of OX2R protein in hypothalamus tissue was examined by immunohistochemical staining (400×，brown-yellow granules indicate positive expression). Data are expressed as the mean ± SD. # P < 0.05, ## P < 0.01 compare with control group; *P < 0.05 and **P < 0.01 compare with model group.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.