製品: PARD6A Antibody
カタログ: DF7166
タンパク質の説明: Rabbit polyclonal antibody to PARD6A
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 37kDa; 37kD(Calculated).
ユニプロット: Q9NPB6
RRID: AB_2839118

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
PARD6A Antibody detects endogenous levels of total PARD6A.
RRID:
AB_2839118
引用形式: Affinity Biosciences Cat# DF7166, RRID:AB_2839118.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

0710008C04Rik; 2610010A15Rik; Par 6 partitioning defective 6 C elegans homolog alpha; Par 6 partitioning defective 6 homolog alpha; Par 6 partitioning defective 6 homolog alpha C elegans; PAR 6A; PAR-6 alpha; PAR-6; par-6 family cell polarity regulator alpha; PAR-6A; PAR6; PAR6A_HUMAN; PAR6alpha; PAR6C; Pard6a; Partitioning defective 6 homolog alpha; partitioning-defective protein 6; partitioning-defective protein 6, C. elegans, homolog of, alpha; Tax interacting protein 40; Tax interaction protein 40; TAX40; TIP 40; TIP-40; TIP40;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9NPB6 PAR6A_HUMAN:

Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.

タンパク質の説明:
This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
タンパク質配列:
MARPQRTPARSPDSIVEVKSKFDAEFRRFALPRASVSGFQEFSRLLRAVHQIPGLDVLLGYTDAHGDLLPLTNDDSLHRALASGPPPLRLLVQKRAEADSSGLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQVSSVIDVDLLPETHRRVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGGLAESTGLLAVSDEILEVNGIEVAGKTLDQVTDMMVANSHNLIVTVKPANQRNNVVRGASGRLTGPPSAGPGPAEPDSDDDSSDLVIENRQPPSSNGLSQGPPCWDLHPGCRHPGTRSSLPSLDDQEQASSGWGSRIRGDGSGFSL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
58
Zebrafish
50
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPB6 基板として

Site PTM Type Enzyme
K19 Ubiquitination
S100 Phosphorylation
S101 Phosphorylation
S107 Phosphorylation
S109 Phosphorylation
S165 Phosphorylation
S278 Phosphorylation
S282 Phosphorylation
S345 Phosphorylation P37173 (TGFBR2) , P41743 (PRKCI)

研究背景

機能:

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization.

PTMs:

Phosphorylated by the TGF-beta receptor.

細胞の位置付け:

Cytoplasm. Cell membrane. Cell projection>Ruffle. Cell junction>Tight junction. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions. Recruited to the centrosome by a microtubule and dynein-dynactin-dependent mechanism.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.

サブユニット構造:

Interacts with MAP2K5 (By similarity). Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI (By similarity). Interacts with human T-cell leukemia virus type I TAX protein. Interacts with MPP5 and CRB3. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with ECT2 ('Thr-359' phosphorylated form) and PRKCI. Interacts with DCTN1 and PCM1.

タンパク質ファミリー:

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PB1 domain mediates interactions with MAP2K5.

The PDZ domain mediates the interaction with CRB3.

Belongs to the PAR6 family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Development > Axon guidance.   (View pathway)

参考文献

1). Constitutive activation of β-catenin in odontoblasts induces aberrant pulp calcification in mouse incisors. JOURNAL OF MOLECULAR HISTOLOGY, 2021 (PubMed: 33689044) [IF=3.2]

Application: WB    Species: Mouse    Sample: calcification tissue

Fig. 6 Downregulation of polarity-related downstream molecules in incisor odontoblasts on day 13.5. a RT-qPCR quantification analy- sis of intercellular junction-related genes and polarity-related down- stream genes. CA-β-catenin mice displayed downregulated expres- sion levels of N-cadherin, ZO-1, Cdc42, Par3, Par6 and aPKC. b Western blot analysis of intercellular junction-related proteins and polarity-related downstream proteins. The fold activation data analy- sis is shown in (c). CA-β-catenin mice exhibited lower expression levels of N-cadherin, ZO-1, Cdc42, Par3, Par6 and aPKC. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6. (Color figure online)

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