Peroxiredoxin 3 Antibody - #DF7174
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7174, RRID:AB_2839126.
折りたたみ／展開
Antioxidant protein 1; AOP 1; AOP-1; AOP1; HBC189; MER5; MGC104387; MGC24293; mitochondrial; peroxiredoxin 3; Peroxiredoxin III; Peroxiredoxin-3; PRDX3; PRDX3_HUMAN; PRO1748; Protein MER5 homolog; PRX III; Prx-III; PRX3; SP 22; SP-22; SP22; Thioredoxin dependent peroxide reductase mitochondrial; Thioredoxin-dependent peroxide reductase;
免疫原
- P30048 PRDX3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P30048 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y71 | Phosphorylation | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K91 | Acetylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K93 | Acetylation | Uniprot | |
| T146 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| K149 | Ubiquitination | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K196 | Sumoylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| R207 | Methylation | Uniprot | |
| R214 | Methylation | Uniprot | |
| C229 | S-Nitrosylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K248 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot |
研究背景
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.
Mitochondrion. Cytoplasm. Early endosome.
Note: Localizes to early endosomes in a RPS6KC1-dependent manner.
Homodimer; disulfide-linked, upon oxidation. 6 homodimers assemble to form a ring-like dodecamer. Interacts with NEK6. Interacts with LRRK2. Interacts with MAP3K13. Interacts with RPS6KC1 (via PX domain).
Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
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