RAD52 Antibody - #DF7175

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製品: RAD52 Antibody
カタログ: DF7175
タンパク質の説明: Rabbit polyclonal antibody to RAD52
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 46kDa; 46kD(Calculated).
ユニプロット: P43351
RRID: AB_2839127

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(89%)
クローナリティ:
Polyclonal
特異性:
RAD52 Antibody detects endogenous levels of total RAD52.
RRID:
AB_2839127
引用形式: Affinity Biosciences Cat# DF7175, RRID:AB_2839127.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DNA repair protein RAD52; DNA repair protein RAD52; DNA repair protein RAD52 homolog; RAD 52; Rad52; RAD52 homolog (S. cerevisiae); RAD52 homolog (S. cerevisiae); RAD52 homolog; RAD52_HUMAN; Recombination protein RAD52; Recombination protein RAD52; Rhabdomyosarcoma antigen MU RMS 40.23;

免疫原

免疫原:
Uniprot:

P43351(RAD52_HUMAN) >>Visit HPA database.

遺伝子(ID):
RAD52(5893)
タンパク質の説明:
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair.
タンパク質配列:
MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVESEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
89
Chicken
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43351 基板として

Site PTM Type Enzyme
S2 Phosphorylation
K41 Ubiquitination
Y104 Phosphorylation P00519 (ABL1) , A0A173G4P4 (Abl fusion) , A9UF07 (BCR/ABL fusion)
S129 Phosphorylation
K133 Acetylation
K135 Ubiquitination
S138 Phosphorylation
K141 Ubiquitination
Y171 Phosphorylation
S174 Phosphorylation
K177 Acetylation
K177 Ubiquitination
K190 Acetylation
K192 Acetylation
S199 Phosphorylation
Y205 Phosphorylation
S207 Phosphorylation
T223 Phosphorylation
S224 Phosphorylation
S251 Phosphorylation
K262 Acetylation
K274 Acetylation
T281 Phosphorylation
K286 Acetylation
S287 Phosphorylation
S299 Phosphorylation
T303 Phosphorylation
T318 Phosphorylation
K323 Acetylation
T335 Phosphorylation
K344 Acetylation
T397 Phosphorylation
K406 Acetylation
K411 Sumoylation
K412 Acetylation
K412 Sumoylation
K414 Acetylation
K414 Sumoylation

研究背景

機能:

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

PTMs:

Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex.

タンパク質ファミリー:

Belongs to the RAD52 family.

研究領域

· Genetic Information Processing > Replication and repair > Homologous recombination.

参考文献

1). LncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple negative breast cancer by regulation of miR-26a-5p/MTDH pathway-mediated autophagy and genomic instability. Aging, 2021 (PubMed: 34740994) [IF=5.2]

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