製品: NEUROG1 Antibody
カタログ: DF7192
タンパク質の説明: Rabbit polyclonal antibody to NEUROG1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit, Dog
分子量: 26kDa; 26kD(Calculated).
ユニプロット: Q92886
RRID: AB_2839144

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(89%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
NEUROG1 Antibody detects endogenous levels of total NEUROG1.
RRID:
AB_2839144
引用形式: Affinity Biosciences Cat# DF7192, RRID:AB_2839144.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AKA; Ath4c; bHLHa6; Class A basic helix-loop-helix protein 6; Helix loop helix protein mATH 4C; MATH4C; NeuroD3; Neurog 1; Neurog1; Neurogenic basic helix loop helix protein; Neurogenic basic-helix-loop-helix protein; Neurogenic differentiation 3; Neurogenic differentiation factor 3; Neurogenin-1; Neurogenin1; Ngn; NGN-1; Ngn1; NGN1_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q92886 NGN1_HUMAN:

Expression restricted to the embryonic nervous system.

タンパク質の説明:
Neurogenin-1 is a protein that in humans is encoded by the NEUROG1 gene. Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. NEUROG1 has been shown to interact with CREB-binding protein and decapentaplegic homolog 1.
タンパク質配列:
MPARLETCISDLDCASSSGSDLSGFLTDEEDCARLQQAASASGPPAPARRGAPNISRASEVPGAQDDEQERRRRRGRTRVRSEALLHSLRRSRRVKANDRERNRMHNLNAALDALRSVLPSFPDDTKLTKIETLRFAYNYIWALAETLRLADQGLPGGGARERLLPPQCVPCLPGPPSPASDAESWGSGAAAASPLSDPSSPAASEDFTYRPGDPVFSFPSLPKDLLHTTPCFIPYH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
89
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92886 基板として

Site PTM Type Enzyme
S178 Phosphorylation Q13164 (MAPK7)
S201 Phosphorylation Q13164 (MAPK7)

研究背景

機能:

Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expression restricted to the embryonic nervous system.

サブユニット構造:

Efficient DNA binding requires dimerization with another bHLH protein.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

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