SLC22A8 Antibody - #DF7196

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製品: SLC22A8 Antibody
カタログ: DF7196
タンパク質の説明: Rabbit polyclonal antibody to SLC22A8
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Rabbit
分子量: 60kDa; 60kD(Calculated).
ユニプロット: Q8TCC7
RRID: AB_2839148

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:25-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
SLC22A8 Antibody detects endogenous levels of total SLC22A8.
RRID:
AB_2839148
引用形式: Affinity Biosciences Cat# DF7196, RRID:AB_2839148.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

hOAT3; OAT3; Organic anion transporter 3; S22A8_HUMAN; SLC22A8; Solute carrier family 22 (organic anion transporter), member 8; Solute carrier family 22 member 8;

免疫原

免疫原:
Uniprot:

Q8TCC7(S22A8_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC22A8(9376)
発現特異性:
Q8TCC7 S22A8_HUMAN:

Expressed in kidney.

タンパク質の説明:
This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
タンパク質配列:
MTFSEILDRVGSMGHFQFLHVAILGLPILNMANHNLLQIFTAATPVHHCRPPHNASTGPWVLPMGPNGKPERCLRFVHPPNASLPNDTQRAMEPCLDGWVYNSTKDSIVTEWDLVCNSNKLKEMAQSIFMAGILIGGLVLGDLSDRFGRRPILTCSYLLLAASGSGAAFSPTFPIYMVFRFLCGFGISGITLSTVILNVEWVPTRMRAIMSTALGYCYTFGQFILPGLAYAIPQWRWLQLTVSIPFFVFFLSSWWTPESIRWLVLSGKSSKALKILRRVAVFNGKKEEGERLSLEELKLNLQKEISLAKAKYTASDLFRIPMLRRMTFCLSLAWFATGFAYYSLAMGVEEFGVNLYILQIIFGGVDVPAKFITILSLSYLGRHTTQAAALLLAGGAILALTFVPLDLQTVRTVLAVFGKGCLSSSFSCLFLYTSELYPTVIRQTGMGVSNLWTRVGSMVSPLVKITGEVQPFIPNIIYGITALLGGSAALFLPETLNQPLPETIEDLENWSLRAKKPKQEPEVEKASQRIPLQPHGPGLGSS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
75
Dog
75
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TCC7 基板として

Site PTM Type Enzyme
S4 Phosphorylation
T439 Phosphorylation

研究背景

機能:

Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).

細胞の位置付け:

Basolateral cell membrane>Multi-pass membrane protein.
Note: Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in kidney.

タンパク質ファミリー:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

参考文献

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE, 2022 (PubMed: 35714456) [IF=7.9]
2). Hypouricemic effect of gallic acid, a bioactive compound from Sonneratia apetala leaves and branches, on hyperuricemic mice. Food & Function, 2022 (PubMed: 36125096) [IF=6.1]
3). Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway. European Journal of Pharmacology, 2021 (PubMed: 34699754) [IF=5.0]
4). Xanthones from securidaca inappendiculata antagonized the anti-rheumatic effects of methotrexate in vivo by promoting its secretion into urine. Expert Opinion on Drug Metabolism & Toxicology, 2021 (PubMed: 33107357) [IF=4.3]

Application: WB    Species: rat    Sample: kidney

Figure 4. | XRF promoted MTX secretion into urine in CIA rats.; B, effects of treatments on OAT3 expression in kidney, investigated by the immunoblotting method, **p < 0.01 compared with CIA models, ##p < 0.01 compared with MTX treated rats
5). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY, 2021 (PubMed: 33651969) [IF=3.8]

Application: WB    Species: Mice    Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3).  p < 0.05,  p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control;  p < 0.01 versus the allopurinol control.
6). CP-25 ameliorates methotrexate induced nephrotoxicity via improving renal apoptosis and methotrexate excretion. Journal of Pharmacological Sciences, 2021 (PubMed: 33858651) [IF=3.5]

Application: WB    Species: Rat    Sample: renal tissue

Fig. 4. Effects of CP-25 on renal OAT3 expression and function in vivo. A: the renal excretion of MTX were measured in rats that received a single intravenous injection of MTX (5 mg/kg). B: the tissue extracts were prepared, and the lysate was resolved by SDS-PAGE and probed with antibodies against OAT3. C: OAT3 function was evaluated by detecting DHEA excretion in the rats received a single intravenous injection of DHEA (20 mg/kg). The data are presented as the means ± S.D. (n ¼ 6). ##P < 0.01 compared with normal group; *P < 0.05, **P < 0.01 compared with the model group (MTX).

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