PARP1 Antibody - #DF7198

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製品: PARP1 Antibody
カタログ: DF7198
タンパク質の説明: Rabbit polyclonal antibody to PARP1
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 89kDa(cleaved), 113kDa(precursor); 113kD(Calculated).
ユニプロット: P09874
RRID: AB_2839150

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HPLC Report
MSDS
説明書
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
PARP1 Antibody detects endogenous levels of total PARP1.
RRID:
AB_2839150
引用形式: Affinity Biosciences Cat# DF7198, RRID:AB_2839150.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ADPRT; ADPRT1; APOPAIN; ARTD1; NAD(+) ADP-ribosyltransferase 1; PARP; PARP-1; PARP1; PARP1_HUMAN; Poly [ADP-ribose] polymerase 1; Poly ADP ribose polymerase 1; Poly[ADP-ribose] synthase 1; PPOL; SCA1;

免疫原

免疫原:

A synthesized peptide derived from human PARP1, corresponding to a region within the internal amino acids.

Uniprot:

P09874(PARP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PARP1(142)
タンパク質の説明:
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
タンパク質配列:
MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Mediates the poly(ADP-ribosyl)ation of a number of proteins, including itself, APLF and CHFR. Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity. Probably also catalyzes tyrosine ADP-ribosylation of target proteins following interaction with HPF1. Catalyzes the poly-ADP-ribosylation of histones in a HPF1-dependent manner. Involved in the base excision repair (BER) pathway by catalyzing the poly-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. In addition to base excision repair (BER) pathway, also involved in double-strand breaks (DSBs) repair: together with TIMELESS, accumulates at DNA damage sites and promotes homologous recombination repair by mediating poly-ADP-ribosylation. In addition to proteins, also able to ADP-ribosylate DNA: catalyzes ADP-ribosylation of DNA strand break termini containing terminal phosphates and a 2'-OH group in single- and double-stranded DNA, respectively. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. Acts as a regulator of transcription: positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.

PTMs:

Phosphorylated by PRKDC and TXK.

Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.

S-nitrosylated, leading to inhibit transcription regulation activity.

細胞の位置付け:

Nucleus. Nucleus>Nucleolus. Chromosome.
Note: Localizes to sites of DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Genetic Information Processing > Replication and repair > Base excision repair.

参考文献

1). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=16.6]

Application: WB    Species: mouse    Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.
2). Cooperative STAT3-NFkB signaling modulates mitochondrial dysfunction and metabolic profiling in hepatocellular carcinoma. Metabolism: clinical and experimental, 2024 (PubMed: 38184165) [IF=10.8]
3). Danhong injection alleviates cerebral ischemia/reperfusion injury by improving intracellular energy metabolism coupling in the ischemic penumbra. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34058441) [IF=6.9]

Application: WB    Species: rat    Sample:

Fig. 4.| Effect of DHI on the activity of PARP1/AIF signaling pathway and the content of molecules associated with cytoplasmic glycolysis. A-E: Representative images of WB analysis and the semi-quantification of PARP1, PAR, AIF, and HSP70. Data are expressed as mean ± SD (n = 3).

Application: IHC    Species: rat    Sample: brain

Fig. 5.| Effect of DHI on the content of molecules associated with cytoplasmic glycolysis. A-F: Immunostaining photomicrographs of PARP1, AIF, HSP70 and quantitative analysis of the IOD.
4). Perilla frutescens L. alleviates trimethylamine N-oxide–induced apoptosis in the renal tubule by regulating ASK1-JNK phosphorylation. Phytotherapy Research, 2023 (PubMed: 36420586) [IF=6.1]
5). Exploring the Critical Components and Therapeutic Mechanisms of Perilla frutescens L. in the Treatment of Chronic Kidney Disease via Network Pharmacology. Frontiers in Pharmacology, 2021 (PubMed: 34899287) [IF=5.6]

Application: WB    Species: Rat    Sample: NRK-52E cells

FIGURE 8 Luteolin reduces ADR-induced apoptosis. (A) Effects of luteolin in ADR-induced apoptosis by TUNEL and DAPI staining assay. NRK-52E cells were incubated with ADR for 24 h in the presence and absence of luteolin. Apoptotic cells were detected by TUNEL and DAPI staining. Fluorescence staining was observed by fluorescence microscope (magnification ×200) and the analysis result on the right was expressed as the percentages of dead cells compared with the Ctrl (mean ± SD, n = 3; *p
6). Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway. Marine Drugs, 2018 (PubMed: 29509717) [IF=5.4]

Application: WB    Species: mouse    Sample:

Figure 5. | Effects on apoptosis-related protein expression, poly ADP ribose polymerase (PARP), and caspase-3 activity in vivo. After pretreated with MPTP for seven days, the C57BL/6 mice were administrated with MA or different concentrations of OP for the followed 7 days. (A): Original bands of cytochrome c (CytC), cleaved caspased-3, B-cell lymphoma 2 (Bcl-2), BCL2 associated X (Bax), PARP, and β-actin.
7). FGFRL1 affects chemoresistance of small-cell lung cancer by modulating the PI3K/Akt pathway via ENO1. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 31957179) [IF=5.3]

Application: WB    Species: Human    Sample: SCLC cells

Figure 3 FGFRL1 induces chemoresistance of SCLC mainly by decreasing drug‐induced apoptosis and cell cycle arrest. A, B, Cell apoptosis and cell cycle arrest were evaluated by flow cytometric analysis in FGFRL1–down‐regulated SCLC cells after ADM exposure. C, D, Flow cytometric analysis of cell apoptosis and cell cycle arrest induced by ADM in FGFRL1‐overexpressing SCLC cells. E, F, Apoptosis‐related proteins were measured by Western blot following anticancer drug exposure in SCLC cells with down‐regulated or up‐regulated FGFRL1 expression. **P < .01; ***P < .001
8). Perillaldehyde targeting PARP1 to inhibit TRPM2-CaMKII/CaN signal transduction in diabetic cardiomyopathy. International immunopharmacology, 2025 (PubMed: 39970708) [IF=4.8]
9). SHMT2 promotes tumor growth through VEGF and MAPK signaling pathway in breast cancer. American Journal of Cancer Research, 2023 (PubMed: 35968337) [IF=3.6]
10). The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer. Frontiers in Oncology, 2020 (PubMed: 33134168) [IF=3.5]

Application: IHC    Species: Human    Sample: tumor tissues

Figure 1 PARP1 mRNA tightly correlates with MYC paralog expression and is an independent prognostic marker of survival in patients with SCLC. (A) Kaplan–Meier analysis of the correlation between PARP1 expression and overall survival (OS, n = 77) and (B) progression-free survival (PFS, n=33). (C–E) Scatter plots of PARP1 mRNA relative to expression of MYC paralogs in SCLC primary tumors (n=81) (C), CCLE cell lines (n=50) (D), murine SCLC tumors (n=14) (E). (F) Representative images of IHC analysis of PARP1 and c-MYC in two independent cases. Scale bar, 100 μm. (G) Heat map showing the correlation between PARP1 and c-MYC in 17 paraffin-embedded SCLC tumor tissues. The heat map was depicted according to the IOD value of each IHC slides (red indicates c-MYC and PARP1 positive staining, green indicates negative staining). The significance analysis was performed by Student’s t test. (H–I) ChIP-qRT-PCR experiment indicating the direct binding of c-MYC and MYCN to the PARP1 promoter in DMS273 (H) and H526 (I) cells. (J) Western blot analysis showing the downregulated proteins in DMS53 and DMS273 cells upon c-MYC knockdown. (K) Western blot analysis showing the upregulated proteins in SHP77 cells with ectopic c-MYC overexpression. GAPDH was used as a loading control. BS, binding site.

Application: WB    Species: Human    Sample: tumor tissues

Figure 1 PARP1 mRNA tightly correlates with MYC paralog expression and is an independent prognostic marker of survival in patients with SCLC. (A) Kaplan–Meier analysis of the correlation between PARP1 expression and overall survival (OS, n = 77) and (B) progression-free survival (PFS, n=33). (C–E) Scatter plots of PARP1 mRNA relative to expression of MYC paralogs in SCLC primary tumors (n=81) (C), CCLE cell lines (n=50) (D), murine SCLC tumors (n=14) (E). (F) Representative images of IHC analysis of PARP1 and c-MYC in two independent cases. Scale bar, 100 μm. (G) Heat map showing the correlation between PARP1 and c-MYC in 17 paraffin-embedded SCLC tumor tissues. The heat map was depicted according to the IOD value of each IHC slides (red indicates c-MYC and PARP1 positive staining, green indicates negative staining). The significance analysis was performed by Student’s t test. (H–I) ChIP-qRT-PCR experiment indicating the direct binding of c-MYC and MYCN to the PARP1 promoter in DMS273 (H) and H526 (I) cells. (J) Western blot analysis showing the downregulated proteins in DMS53 and DMS273 cells upon c-MYC knockdown. (K) Western blot analysis showing the upregulated proteins in SHP77 cells with ectopic c-MYC overexpression. GAPDH was used as a loading control. BS, binding site.
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