SLC5A1 Antibody - #DF7202

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製品: SLC5A1 Antibody
カタログ: DF7202
タンパク質の説明: Rabbit polyclonal antibody to SLC5A1
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 60kDa; 73kD(Calculated).
ユニプロット: P13866
RRID: AB_2839154

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Zebrafish(83%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(83%), Dog(92%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
SLC5A1 Antibody detects endogenous levels of total SLC5A1.
RRID:
AB_2839154
引用形式: Affinity Biosciences Cat# DF7202, RRID:AB_2839154.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

D22S675; High affinity sodium glucose cotransporter 1; High affinity sodium glucose cotransporter; High affinity sodium-glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; Na+/glucose cotransporter 1; NAGT; SC5A1_HUMAN; SGLT 1; SGLT1; SLC5A1; Sodium glucose cotransporter 1; Sodium/glucose cotransporter 1; Solute carrier family 5 (sodium/glucose cotransporter) member 1; Solute carrier family 5 member 1;

免疫原

免疫原:
Uniprot:

P13866(SC5A1_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC5A1(6523)
発現特異性:
P13866 SC5A1_HUMAN:

Expressed mainly in intestine and kidney.

タンパク質の説明:
This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.
タンパク質配列:
MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Pig
92
Dog
92
Chicken
92
Horse
83
Zebrafish
83
Rabbit
83
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13866 基板として

Site PTM Type Enzyme
Y236 Phosphorylation
T242 Phosphorylation
N248 N-Glycosylation
T271 Phosphorylation
S335 Phosphorylation
S418 Phosphorylation
S437 Phosphorylation
T587 Phosphorylation
Y606 Phosphorylation

研究背景

機能:

Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

PTMs:

N-glycosylation is not necessary for the cotransporter function.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed mainly in intestine and kidney.

タンパク質ファミリー:

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

研究領域

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

参考文献

1). Xinbao Pill ameliorates heart failure via regulating the SGLT1/AMPK/PPARα axis to improve myocardial fatty acid energy metabolism. Chinese medicine, 2024 (PubMed: 38862959) [IF=4.9]
2). Phlorizin Exerts Direct Protective Effects on Palmitic Acid (PA)-Induced Endothelial Dysfunction by Activating the PI3K/AKT/eNOS Signaling Pathway and Increasing the Levels of Nitric Oxide (NO). Medical Science Monitor Basic Research, 2018 (PubMed: 29307883) [IF=2.8]

Application: WB    Species: human    Sample: HUVECs

Figure 1.| PA stimulates expression of SGLT1 and SGLT2 in HUVECs. Cells were incubated for 18 h in the presence of PA (300 µM). (A, B) SGLT1 and SGLT2 proteins in the samples were analyzed by Western blotting. Bar plots show the summarized data of the relative density after being normalized to GAPDH and are expressed as average percentage of control ±SEM. (C, D) Quantitative PCR analysis of mRNA expression of SGLT1 and SGLT2, normalized to GAPDH mRNA. ** P<0.01.

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