DNMT3A Antibody - #DF7226

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製品: DNMT3A Antibody
カタログ: DF7226
タンパク質の説明: Rabbit polyclonal antibody to DNMT3A
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 120-140kD; 102kD(Calculated).
ユニプロット: Q9Y6K1
RRID: AB_2839167

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MS Report
HPLC Report
MSDS
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(82%), Bovine(91%), Horse(82%), Rabbit(91%), Dog(82%)
クローナリティ:
Polyclonal
特異性:
DNMT3A Antibody detects endogenous levels of total DNMT3A.
RRID:
AB_2839167
引用形式: Affinity Biosciences Cat# DF7226, RRID:AB_2839167.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DNA (cytosine 5) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3 alpha; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNM3A_HUMAN; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M.HsaIIIA; MCMT; OTTHUMP00000201149; TBRS;

免疫原

免疫原:

A synthesized peptide derived from human DNMT3A, corresponding to a region within the internal amino acids.

Uniprot:

Q9Y6K1(DNM3A_HUMAN) >>Visit HPA database.

遺伝子(ID):
DNMT3A(1788)
発現特異性:
Q9Y6K1 DNM3A_HUMAN:

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

タンパク質の説明:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.
タンパク質配列:
MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAGMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAVEVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Bovine
91
Pig
82
Horse
82
Dog
82
Chicken
64
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

PTMs:

Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Accumulates in the major satellite repeats at pericentric heterochromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

タンパク質ファミリー:

The PWWP domain is essential for targeting to pericentric heterochromatin. It specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3) (By similarity).

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

研究領域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Restoration of mitochondrial function alleviates trigeminal neuropathic pain in mice. Free radical biology & medicine, 2025 (PubMed: 39528053) [IF=7.4]
2). Total Barley Maiya Alkaloids Prevent Increased Prolactin Levels Caused by Antipsychotic Drugs and Reduce Dopamine Receptor D2 via Epigenetic Mechanisms. Frontiers in Pharmacology, 2022 (PubMed: 35865960) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 4 Effects of TBMA, hordenine, and N-methyltyramine on DRD2 and DNMT expression. (A–E) DRD2, DNMT1, DNMT3α, and DNMT3β protein expression. GAPDH was used as a loading control. (F) DRD2, DNMT1, DNMT3α, and DNMT3β mRNA expression. Data are presented as the mean ± SD. Protein expression in each group was determined using western blotting. mRNA expression in each group was determined using RT-PCR (n = 3 rats per group; # p < 0.05 and ## p < 0.01 versus the control group; * p < 0.05 versus the Model group. Bro = bromocriptine; Hor = hordenine; N-Methy = N-methyltyramine; Ris = risperidone; TBMA = total barley maiya alkaloids.
3). Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals. International journal of molecular sciences, 2024 (PubMed: 38473715) [IF=5.6]

Application: WB    Species: Mouse    Sample: GCs

Figure 5. Knockdown of DNMT1 upregulates the mRNA and protein levels of SLCO3A1. (A) RT-qPCR analysis of SLCO3A1 expression in the small (3 mm in diameter) follicles. (B) Western blot analysis of SLCO3A1 in the small and large follicles. (C) RT-qPCR analysis of SLCO3A1 expression in the DMSO or 5-Aza-CdR treated-cells. (D) Western blot analysis of SLCO3A1 in the DMSO or 5-Aza-CdR treated-cells. (E) Chromatin accessibility assay of region-1, region-2, and region-3 in the SLCO3A1 promoter treated with DMSO or 5-Aza-CdR (region-1, +971/+1252 bp, region-2, +1433/+1557 bp, and region-3, +1623/+1831 bp, transcription start site = +2000 bp). (F) RT-qPCR analysis of SLCO3A1 expression in GCs transfected with siRNAs of DNMT1, DNMT3A, or DNMT3B. Western blot analysis of SLCO3A1, DNMT1 (G), DNMT3A (H), and DNMT3B (I) in the siRNAs of DNMT1, DNMT3A, or DNMT3B treated-cells. *** p < 0.001.
4). Decreased ovarian function and autophagy gene methylation in aging rats. Journal of Ovarian Research, 2020 (PubMed: 32014030) [IF=4.0]

Application: WB    Species: rat    Sample: ovaries

Fig. 6| Protein expression of Dnmt1, Dnmt2, Dnmt3A and Dnmt3B in the ovaries of rats.(a) Western blot results.
5). Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2021 (PubMed: 33011952) [IF=3.5]

Application: WB    Species: human    Sample: HCT-8 and HCT-8/Fu cells

Fig. 4 |Efects of BPIS on the expression of DNA methylation-related enzymes. a, b The expressions of DNMT3a, DNMT3b and MECP2 were measured in RNA and protein levels in HCT-8 and HCT-8/Fu cells by RT-PCR and western blotting assays.
6). Prenatal stress increases corticosterone levels in offspring by impairing placental glucocorticoid barrier function. Research Square, 2023

Application: WB    Species: Rat    Sample:

Figure 4. Effects of prenatal stress on offspring enzymes of DNA methylation in placenta. The figure showed that the expressions of DNMT1, DNMT 3A, DNMT 3B, and β-actin were identified by q-PCR and western blot. The levels of DNMT 3A and DNMT 3B were reduced but DNMT1 expression was enhanced by prenatal stress on offspring. Three biological replicates were carried out. DNMT1, DNA Methyltransferase 1; DNMT 3A, DNA Methyltransferase 3A; DNMT 3B, DNA Methyltransferase 3B. All data are shown as mean ± SD. Number of animals in each group = 3. *Statistically significant difference compared with the OPC group (P

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