製品: Nono Antibody
カタログ: DF7254
タンパク質の説明: Rabbit polyclonal antibody to Nono
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 54kDa; 54kD(Calculated).
ユニプロット: Q15233
RRID: AB_2839193

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Nono Antibody detects endogenous levels of total Nono.
RRID:
AB_2839193
引用形式: Affinity Biosciences Cat# DF7254, RRID:AB_2839193.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

52 kDa subunit; 54 kDa nuclear RNA and DNA binding protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA binding p52/p100 complex 52 kDa subunit; DNA-binding p52/p100 complex; NMT 55; NMT55; Non Pou domain containing octamer (ATGCAAAT) binding protein; Non POU domain containing octamer binding; Non POU domain containing octamer binding protein; Non-POU domain-containing octamer-binding protein; Nono; NonO protein; NONO_HUMAN; NRB 54; NRB; NRB54; Nuclear RNA binding protein 54kD; P54; p54(nrb); p54nrb; PPP1R114; Protein phosphatase 1 regulatory subunit 114;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q15233 NONO_HUMAN:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

タンパク質の説明:
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
タンパク質配列:
MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
70
Chicken
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15233 基板として

Site PTM Type Enzyme
K5 Acetylation
K5 Methylation
K5 Sumoylation
K5 Ubiquitination
T6 Phosphorylation
K11 Acetylation
K11 Sumoylation
K11 Ubiquitination
T15 Phosphorylation
K60 Sumoylation
K60 Ubiquitination
K64 Ubiquitination
K68 Acetylation
K68 Sumoylation
K68 Ubiquitination
T86 Phosphorylation
K92 Ubiquitination
K96 Acetylation
K96 Sumoylation
K96 Ubiquitination
K99 Sumoylation
K99 Ubiquitination
K107 Acetylation
K107 Sumoylation
K107 Ubiquitination
K109 Acetylation
K109 Sumoylation
K109 Ubiquitination
R115 Methylation
K126 Acetylation
K126 Sumoylation
K126 Ubiquitination
C145 S-Nitrosylation
S147 Phosphorylation
S149 Phosphorylation
T151 Phosphorylation
R184 Methylation
R186 Methylation
S188 Phosphorylation
K190 Acetylation
K190 Methylation
K190 Sumoylation
K190 Ubiquitination
K198 Acetylation
K198 Sumoylation
K198 Ubiquitination
S209 Phosphorylation
S212 Phosphorylation
K239 Sumoylation
K239 Ubiquitination
K243 Acetylation
K243 Sumoylation
K243 Ubiquitination
K249 Ubiquitination
R256 Methylation
S262 Phosphorylation
Y265 Phosphorylation
Y267 Phosphorylation
K272 Sumoylation
K272 Ubiquitination
K279 Sumoylation
K279 Ubiquitination
R287 Methylation
K290 Sumoylation
K290 Ubiquitination
K295 Sumoylation
K295 Ubiquitination
R304 Methylation
R325 Methylation
K336 Ubiquitination
K338 Ubiquitination
R343 Methylation
R364 Methylation
K371 Acetylation
K371 Sumoylation
K371 Ubiquitination
T373 Phosphorylation
T410 Phosphorylation
T418 Phosphorylation
T424 Phosphorylation
T428 Phosphorylation
T431 Phosphorylation
T432 Phosphorylation
T440 Phosphorylation
T450 Phosphorylation
R456 Methylation
K467 Acetylation
K467 Methylation
K467 Sumoylation
K467 Ubiquitination
Y471 Phosphorylation

研究背景

機能:

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

PTMs:

The N-terminus is blocked.

細胞の位置付け:

Nucleus. Nucleus>Nucleolus. Nucleus speckle.
Note: Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

サブユニット構造:

Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with CPNE4 (via VWFA domain) (By similarity). Forms heterodimers with PSPC1; this involves formation of a coiled coil domain by helices from both proteins. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ, NONO and NR5A1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1 (By similarity). Interacts with RNF43. Interacts with PER1 and PER2 (By similarity). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts (via second RRM domain) with WASL; the interaction is direct. Component of a multiprotein complex with WASL and SFPQ. Interacts with ERCC6.

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