GCLM Antibody - #DF7268

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製品: GCLM Antibody
カタログ: DF7268
タンパク質の説明: Rabbit polyclonal antibody to GCLM
アプリケーション: WB IHC
反応性: Human, Mouse, Rat, Pig
予測: Pig, Bovine, Rabbit
分子量: 30kDa; 31kD(Calculated).
ユニプロット: P48507
RRID: AB_2839207

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Pig
予測:
Bovine(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
GCLM Antibody detects endogenous levels of total GCLM.
RRID:
AB_2839207
引用形式: Affinity Biosciences Cat# DF7268, RRID:AB_2839207.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Gamma ECS regulatory subunit; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCLM; GCS light chain; GLCLR; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase modifier subunit; Glutamate--cysteine ligase regulatory subunit; GSC light chain; GSH0_HUMAN;

免疫原

免疫原:
Uniprot:

P48507(GSH0_HUMAN) >>Visit HPA database.

遺伝子(ID):
GCLM(2730)
発現特異性:
P48507 GSH0_HUMAN:

In all tissues examined. Highest levels in skeletal muscle.

タンパク質の説明:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
タンパク質配列:
MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Rabbit
100
Xenopus
78
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48507 基板として

Site PTM Type Enzyme
K9 Sumoylation
K9 Ubiquitination
T17 Phosphorylation
K34 Ubiquitination
C35 S-Nitrosylation
K49 Ubiquitination
C72 S-Nitrosylation
K80 Ubiquitination
K90 Ubiquitination
K158 Sumoylation
K158 Ubiquitination
T164 Phosphorylation
S165 Phosphorylation
K169 Ubiquitination
K205 Sumoylation
K263 Acetylation
K263 Ubiquitination
Y265 Phosphorylation
K270 Ubiquitination

研究背景

組織特異性:

In all tissues examined. Highest levels in skeletal muscle.

サブユニット構造:

Heterodimer of a catalytic heavy chain and a regulatory light chain.

タンパク質ファミリー:

Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

研究領域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress. FOOD RESEARCH INTERNATIONAL, 2021 (PubMed: 33509464) [IF=8.1]
2). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE, 2022 (PubMed: 36150346) [IF=7.9]
3). Itaconate inhibits ferroptosis of macrophage via Nrf2 pathways against sepsis-induced acute lung injury. Cell Death Discovery, 2022 (PubMed: 35110526) [IF=7.0]

Application: WB    Species: Mice    Sample: lung tissues

Fig. 3 4-OI inhibits ferroptosis through increasing and activating Nrf2 in vivo during ALI. A Western blots for Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue. B–E Relative mRNA levels of Nrf2, GCLM, HO-1 and SLC7A11 in murine lung tissue (n = 6). F, G Relative levels of GSH and GSH/GSSG ratio in murine lung tissue (n = 6). H, I Representative images of fluorescence probe for ROS and its statistical results (n = 6) in lung tissue. (Data are presented as Mean ± SD *p < 0.05, **p < 0.01, ***p < 0.001).
4). Ameliorate effect of pyrroloquinoline quinone against cyclophosphamide-induced nephrotoxicity by activating the Nrf2 pathway and inhibiting the NLRP3 pathway. LIFE SCIENCES, 2020 (PubMed: 32504759) [IF=6.1]

Application: WB    Species: Mice    Sample: kidney tissues

Fig. 6. Effect of PQQ on protein expression in the Nrf2/HO-1 pathway. The expression levels of Nrf2 (A), HO-1 (B), GCLM (C), and NQO1 (D). Data are shown as mean ± SD, n = 8. #p < 0.05 and ##p < 0.01 versus the Control group. ⁎ p < 0.05 and ⁎⁎p < 0.01 versus the Model group.
5). Therapeutic role of D-pinitol on experimental colitis via activating Nrf2/ARE and PPAR-γ/NF-κB signaling pathways. Food & Function, 2021 (PubMed: 33625409) [IF=6.1]
6). Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide. Environmental Science and Pollution Research, 2022 (PubMed: 34997497) [IF=5.8]
7). Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug. International immunopharmacology, 2021 (PubMed: 34628270) [IF=5.6]

Application: WB    Species: Rat    Sample: liver tissues

Fig. 6. Curc-mPEG454 up-regulates GCLC and GCLM for GSH de novo synthesis. (A) Heatmap represents gene expression of Glutathione metabolism. Gene related to glutathione synthesis and metabolism are indicated. (B) Paired display the gene expression by RNA-seq and relative mRNA expression by qRT-PCR from glutathione synthesis and metabolism. Gene expression level was measured in FPKM value. The mRNA levels were normalized to GAPDH, and subsequently represents as fold change relative to control group. (C) The protein levels of NRF2, GCLC and GCLM. (D) Curc-mPEG454-100 mg significantly restores GSH level during liver fibrosis. Contents of GSH in liver tissues were detected at the 6th week. Data are expressed as mean ± SEM. #P < 0.05, ##P < 0.01, ###P < 0.001 compared with normal group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with CCl4-induced model group.
8). Hepatoprotective and Anti-Oxidative Effects of Total Flavonoids From Qu Zhi Qiao (Fruit of Citrus Paradisi cv.Changshanhuyou) on Nonalcoholic Steatohepatitis In Vivo and In Vitro Through Nrf2-ARE Signaling Pathway. Frontiers in Pharmacology, 2020 (PubMed: 32390839) [IF=5.6]

Application: WB    Species: mouse    Sample: liver

FIGURE 5 | Expression of the targeted proteins in mice liver. Values are shown as mean ± SD (n = 8). Data (mean ± SD) with different case letters are statistically different with each other at LSD multiple comparisons, which with red letters differed significantly as compared to model group level. uppercase letter p < 0.01 vs.another group.
9). Novel amphibian-derived antioxidant peptide protects skin against ultraviolet irradiation damage. Journal of photochemistry and photobiology-B, Biology, 2021 (PubMed: 34628205) [IF=5.4]
10). Sodium Danshensu Cream Promotes the Healing of Pressure Ulcers in Mice through the Nrf2/HO-1 and NF-κB Pathways. Pharmaceuticals, 2022 (PubMed: 36558999) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 6. Effect of SDSS on the protein levels of the Nrf2/HO-1 pathway (n = 5). (A) Western blot; the protein expression levels of Keap1 (B), Nrf2 (C), HO-1 (D), NQO1 (E), and GCLM (F) levels. * p < 0.05, ** p < 0.01 when compared to the control group. # p < 0.05, ## p < 0.01 when compared to the positive group.
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