Lumican Antibody - #DF7293

(1)   (2)  
製品: Lumican Antibody
カタログ: DF7293
タンパク質の説明: Rabbit polyclonal antibody to Lumican
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
分子量: 38kDa; 38kD(Calculated).
ユニプロット: P51884
RRID: AB_2839232

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MS Report
HPLC Report
MSDS
説明書
COA
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Lumican Antibody detects endogenous levels of total Lumican.
RRID:
AB_2839232
引用形式: Affinity Biosciences Cat# DF7293, RRID:AB_2839232.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Keratan sulfate proteoglycan lumican; KSPG lumican; LDC; LUM; LUM_HUMAN; Lumican; Lumican precursor; SLRR2D;

免疫原

免疫原:
Uniprot:

P51884(LUM_HUMAN) >>Visit HPA database.

遺伝子(ID):
LUM(4060)
発現特異性:
P51884 LUM_HUMAN:

Cornea and other tissues.

タンパク質の説明:
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair.
タンパク質配列:
MSLSAFTLFLALIGGTSGQYYDYDFPLSIYGQSSPNCAPECNCPESYPSAMYCDELKLKSVPMVPPGIKYLYLRNNQIDHIDEKAFENVTDLQWLILDHNLLENSKIKGRVFSKLKQLKKLHINHNNLTESVGPLPKSLEDLQLTHNKITKLGSFEGLVNLTFIHLQHNRLKEDAVSAAFKGLKSLEYLDLSFNQIARLPSGLPVSLLTLYLDNNKISNIPDEYFKRFNALQYLRLSHNELADSGIPGNSFNVSSLVELDLSYNKLKNIPTVNENLENYYLEVNQLEKFDIKSFCKILGPLSYSKIKHLRLDGNRISETSLPPDMYECLRVANEVTLN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
90
Rabbit
71
Xenopus
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51884 基板として

Site PTM Type Enzyme
N88 N-Glycosylation
S138 Phosphorylation
T145 Phosphorylation
N160 N-Glycosylation
S177 Phosphorylation
K181 Acetylation
S192 Phosphorylation
S201 Phosphorylation
T209 Phosphorylation
Y211 Phosphorylation
Y224 Phosphorylation
K226 Acetylation
Y233 Phosphorylation
S304 Phosphorylation
S320 Phosphorylation

研究背景

PTMs:

Sulfated on tyrosine residue(s).

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Cornea and other tissues.

サブユニット構造:

Binds to laminin.

タンパク質ファミリー:

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

研究領域

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

参考文献

1). Stiffness-dependent dynamic effect of inflammation on keratocyte phenotype and differentiation. Biomedical Materials, 2023 (PubMed: 37068490) [IF=4.0]

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