PSME1 Antibody - #DF7298

(3)  
製品: PSME1 Antibody
カタログ: DF7298
タンパク質の説明: Rabbit polyclonal antibody to PSME1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit
分子量: 28kDa; 29kD(Calculated).
ユニプロット: Q06323
RRID: AB_2839237

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
PSME1 Antibody detects endogenous levels of total PSME1.
RRID:
AB_2839237
引用形式: Affinity Biosciences Cat# DF7298, RRID:AB_2839237.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

11S regulator complex alpha subunit; 11S regulator complex subunit alpha; 29 kD MCP activator subunit; 29kD MCP activator subunit; Activator of multicatalytic protease subunit 1; IFI5111; IGUP I-5111; Interferon gamma IEF SSP 5111; Interferon gamma inducible protein 5111; Interferon gamma up regulated I 5111 protein; Interferon gamma up-regulated I-5111 protein; MGC8628; PA28a; PA28alpha; Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; Proteasome activator subunit 1; PSME1; PSME1_HUMAN; REG-alpha; REGalpha;

免疫原

免疫原:
Uniprot:

Q06323(PSME1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PSME1(5720)
タンパク質の説明:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants.
タンパク質配列:
MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Zebrafish
73
Xenopus
45
Horse
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06323 基板として

Site PTM Type Enzyme
K13 Acetylation
K13 Ubiquitination
T23 Phosphorylation
K24 Ubiquitination
T25 Phosphorylation
S31 Phosphorylation
K35 Acetylation
K35 Ubiquitination
K36 Ubiquitination
S38 Phosphorylation
K45 Ubiquitination
S55 Phosphorylation
K58 Sumoylation
K58 Ubiquitination
K90 Acetylation
K109 Ubiquitination
K155 Ubiquitination
S175 Phosphorylation
K176 Ubiquitination
K187 Ubiquitination
K190 Ubiquitination
Y209 Phosphorylation
R213 Methylation
R220 Methylation
K232 Acetylation
K245 Ubiquitination
Y249 Phosphorylation

研究背景

機能:

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

サブユニット構造:

Heterodimer of PSME1 and PSME2, which forms a hexameric ring. PSME1 can form homoheptamers.

タンパク質ファミリー:

Belongs to the PA28 family.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

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