DBI Antibody - #DF7307

(1)  
製品: DBI Antibody
カタログ: DF7307
タンパク質の説明: Rabbit polyclonal antibody to DBI
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 10kDa; 10kD(Calculated).
ユニプロット: P07108
RRID: AB_2839245

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(92%), Bovine(92%), Horse(83%), Sheep(92%), Rabbit(83%), Chicken(83%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
DBI Antibody detects endogenous levels of total DBI.
RRID:
AB_2839245
引用形式: Affinity Biosciences Cat# DF7307, RRID:AB_2839245.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ACBD 1; ACBD1; ACBP; ACBP_HUMAN; Acyl CoA binding domain containing 1; Acyl CoA binding protein; Acyl Coenzyme A binding domain containing 1; Acyl coenzyme A binding protein; Acyl-CoA-binding protein; CCK RP; CCKRP; Cholecystokinin releasing peptide trypsin sensitive; DBI; Diazepam-binding inhibitor; Endozepine; EP; GABA receptor modulator; MGC70414;

免疫原

免疫原:
Uniprot:

P07108(ACBP_HUMAN) >>Visit HPA database.

遺伝子(ID):
DBI(1622)
発現特異性:
P07108 ACBP_HUMAN:

Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

タンパク質の説明:
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene.
タンパク質配列:
MSQAEFEKAAEEVRHLKTKPSDEEMLFIYGHYKQATVGDINTERPGMLDFTGKAKWDAWNELKGTSKEDAMKAYINKVEELKKKYGI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Sheep
92
Zebrafish
92
Horse
83
Xenopus
83
Chicken
83
Rabbit
83
Pig
75
Dog
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07108 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K8 Acetylation
K17 Acetylation
K19 Acetylation
Y29 Phosphorylation
Y32 Phosphorylation
T42 Phosphorylation Q05655 (PRKCD)
T51 Phosphorylation
K53 Ubiquitination
K55 Acetylation
K55 Ubiquitination
K63 Ubiquitination
T65 Phosphorylation
S66 Phosphorylation
K77 Acetylation
K77 Ubiquitination
K82 Ubiquitination

研究背景

機能:

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

細胞の位置付け:

Endoplasmic reticulum. Golgi apparatus.
Note: Golgi localization is dependent on ligand binding (PubMed:17953517).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the ACBP family.

研究領域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

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