PA2G4 Antibody - #DF7311
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7311, RRID:AB_2839249.
折りたたみ／展開
38kDa; AA672939; Cell cycle protein p38 2G4 homolog; Cell cycle protein p38-2G4 homolog; ErbB-3 binding protein 1; ErbB3 binding protein 1; ErbB3-binding protein 1; ErbB3-binding protein Ebp1; hG4 1; hG4-1; IRES-specific cellular trans-acting factor 45 kDa; MGC81621; MGC94070; Mpp1; p38 2G4; Pa2g4; PA2G4_HUMAN; Plfap; Proliferation associated 2G4; Proliferation associated 2G4, 38-KD; Proliferation-associated 2G4, 38kDa; Proliferation-associated 2G4, a; Proliferation-associated protein 1; Proliferation-associated protein 2G4; Protein p38-2G4; si:dz150i12.2; wu:fb19b11; wu:ft56d05; zgc:86732;
免疫原
Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.
- Q9UQ80 PA2G4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UQ80 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| R30 | Methylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| K93 | Sumoylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K172 | Methylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K199 | Methylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| T206 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K260 | Methylation | Uniprot | |
| T261 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| S267 | Phosphorylation | Uniprot | |
| R271 | Methylation | Uniprot | |
| R281 | Methylation | Uniprot | |
| K287 | Acetylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K298 | Acetylation | Uniprot | |
| K298 | Sumoylation | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| K344 | Sumoylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| S360 | Phosphorylation | P17252 (PRKCA) , Q05655 (PRKCD) | Uniprot |
| S361 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | P05129 (PRKCG) , P17252 (PRKCA) , P05771 (PRKCB) | Uniprot |
| T366 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) , P05129 (PRKCG) | Uniprot |
| K373 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| T382 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| T386 | Phosphorylation | Uniprot |
研究背景
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).
Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.
Cytoplasm. Nucleus>Nucleolus.
Note: Translocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
Cytoplasm.
Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.
Isoform 2 interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with NCL/nucleolin. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation. Interacts with AKT1 (By similarity). Isoform 1 and isoform 2 interact with RNF20. Isoform 2 interacts with HUWE1. Interacts with DNAJC21.
Belongs to the peptidase M24 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.