NPC2 Antibody - #DF7331
| 製品: | NPC2 Antibody |
| カタログ: | DF7331 |
| タンパク質の説明: | Rabbit polyclonal antibody to NPC2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Horse, Dog |
| 分子量: | 17kDa; 17kD(Calculated). |
| ユニプロット: | P61916 |
| RRID: | AB_2839269 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7331, RRID:AB_2839269.
折りたたみ／展開
EDDM1; Epididymal protein 1; Epididymal secretory protein; Epididymal secretory protein E1; hE1; Human epididymis-specific protein 1; Niemann-Pick disease type C2; Niemann-Pick disease type C2 protein; NPC intracellular cholesterol transporter 2; NPC2; NPC2_HUMAN; Tissue specific secretory protein;
免疫原
Detected in gallbladder bile (PubMed:21315718). Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level) (PubMed:11125141). Epididymis.
- P61916 NPC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRFLAATFLLLALSTAAQAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVSHL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P61916 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| N58 | N-Glycosylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| N135 | N-Glycosylation | Uniprot |
研究背景
Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).
Secreted. Endoplasmic reticulum. Lysosome.
Note: Interaction with cell-surface M6PR mediates endocytosis and targeting to lysosomes.
Detected in gallbladder bile. Detected in fibroblasts, kidney, liver, spleen, small intestine, placenta and testis (at protein level). Epididymis.
Interacts with NPC1 (via the second lumenal domain) in a cholestrol-dependent manner. Interacts with NUS1/NgBR, the interaction stabilizes NCP2 and regulates cholesterol trafficking. Interacts with DHDDS. Interacts with NEDD4L (via C2 domain). Interacts with NPC1L1.
Binds cholesterol in a hydrophobic pocket; there are no hydrogen bonds between the sterol and the protein.
Belongs to the NPC2 family.
研究領域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Organismal Systems > Digestive system > Cholesterol metabolism.
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