PCCB Antibody - #DF7332
| 製品: | PCCB Antibody |
| カタログ: | DF7332 |
| タンパク質の説明: | Rabbit polyclonal antibody to PCCB |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 58kDa; 58kD(Calculated). |
| ユニプロット: | P05166 |
| RRID: | AB_2839270 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7332, RRID:AB_2839270.
折りたたみ／展開
DKFZp451E113; PCCase subunit beta; pccB; PCCB_HUMAN; pccBC Complementation group; Propanoyl CoA:carbon dioxide ligase subunit beta; Propanoyl-CoA:carbon dioxide ligase subunit beta; Propionyl CoA carboxylase beta chain, mitochondrial; propionyl Coenzyme A carboxylase, beta polypeptide; Propionyl-CoA carboxylase , beta subunit; Propionyl-CoA carboxylase beta chain, mitochondrial; R74805;
免疫原
- P05166 PCCB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAALRVAAVGARLSVLASGLRAAVRSLCSQATSVNERIENKRRTALLGGGQRRIDAQHKRGKLTARERISLLLDPGSFVESDMFVEHRCADFGMAADKNKFPGDSVVTGRGRINGRLVYVFSQDFTVFGGSLSGAHAQKICKIMDQAITVGAPVIGLNDSGGARIQEGVESLAGYADIFLRNVTASGVIPQISLIMGPCAGGAVYSPALTDFTFMVKDTSYLFITGPDVVKSVTNEDVTQEELGGAKTHTTMSGVAHRAFENDVDALCNLRDFFNYLPLSSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P05166 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T45 | Phosphorylation | Uniprot | |
| K60 | Acetylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| Y486 | Phosphorylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| K526 | Acetylation | Uniprot |
研究背景
This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
Mitochondrion matrix.
The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits.
The beta subunit contains the carboxyl transferase (CT) domain.
Belongs to the AccD/PCCB family.
研究領域
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
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