製品: PNMT Antibody
カタログ: DF7393
タンパク質の説明: Rabbit polyclonal antibody to PNMT
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Horse
分子量: 30kDa; 31kD(Calculated).
ユニプロット: P11086
RRID: AB_2839331

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Horse(82%)
クローナリティ:
Polyclonal
特異性:
PNMT Antibody detects endogenous levels of total PNMT.
RRID:
AB_2839331
引用形式: Affinity Biosciences Cat# DF7393, RRID:AB_2839331.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Noradrenaline N-methyltransferase; PENT; Phenylethanolamine N-methyltransferase; PNMT; PNMT_HUMAN; PNMTase;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene.
タンパク質配列:
MSGADRSPNAGAAPDSAPGQAAVASAYQRFEPRAYLRNNYAPPRGDLCNPNGVGPWKLRCLAQTFATGEVSGRTLIDIGSGPTVYQLLSACSHFEDITMTDFLEVNRQELGRWLQEEPGAFNWSMYSQHACLIEGKGECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPLPADALVSAFCLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
82
Pig
73
Bovine
73
Sheep
73
Dog
73
Rabbit
73
Xenopus
50
Chicken
36
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11086 基板として

Site PTM Type Enzyme
S7 Phosphorylation
Y35 Phosphorylation
Y40 Phosphorylation
Y248 Phosphorylation
T255 Phosphorylation
Y256 Phosphorylation

研究背景

機能:

Converts noradrenaline to adrenaline.

タンパク質ファミリー:

Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.

研究領域

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.