MCM5 Antibody - #DF7396
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7396, RRID:AB_2839334.
折りたたみ／展開
CDC 46; CDC46; CDC46 homolog; Cell division cycle 46; DNA replication licensing factor; DNA replication licensing factor MCM 5; DNA replication licensing factor MCM5; MCM 5; MCM5; MCM5_HUMAN; MGC5315; Minichromosome maintenance complex component 5; Minichromosome maintenance deficient (S. cerevisiae) 5; Minichromosome maintenance deficient 5; Minichromosome maintenance deficient protein 5; P1 CDC46; P1-CDC46;
免疫原
- P33992 MCM5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P33992 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| Y212 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| R251 | Methylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| R280 | Methylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| Y332 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| K387 | Acetylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K392 | Acetylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K396 | Acetylation | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| C482 | S-Nitrosylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| R528 | Methylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K581 | Acetylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| S604 | Phosphorylation | Uniprot | |
| S605 | Phosphorylation | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| T633 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S693 | Phosphorylation | Uniprot | |
| K696 | Acetylation | Uniprot | |
| K696 | Ubiquitination | Uniprot | |
| K702 | Ubiquitination | Uniprot |
研究背景
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.
Nucleus. Cytoplasm>Cytosol.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with ANKRD17.
Belongs to the MCM family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.