PIAS2 Antibody - #DF7440
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7440, RRID:AB_2839378.
折りたたみ／展開
Androgen receptor interacting protein 3; Androgen receptor-interacting protein 3; ARIP3; DAB2 interacting protein; DAB2-interacting protein; DIP; E3 SUMO protein ligase PIAS2; E3 SUMO-protein ligase PIAS2; MIZ; Miz1; Msx interacting zinc finger protein; Msx-interacting zinc finger protein; PIAS NY protein; PIAS-NY protein; PIAS2; PIAS2_HUMAN; PIASX; PIASX-ALPHA; PIASX-BETA; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT, 2; Protein inhibitor of activated STAT2; SIZ2; Zinc finger, MIZ-type containing 4; ZMIZ4;
免疫原
Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
- O75928 PIAS2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIHSLPSTSVTPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPISSMSSDLPGLDFLSLIPVDPQYCPPMFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75928 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Q15759 (MAPK11) | Uniprot |
| S116 | Phosphorylation | Q15759 (MAPK11) | Uniprot |
| K127 | Sumoylation | Uniprot | |
| K162 | Sumoylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K249 | Sumoylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K326 | Sumoylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| K350 | Sumoylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K380 | Sumoylation | Uniprot | |
| K430 | Sumoylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K443 | Sumoylation | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| K452 | Sumoylation | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| K464 | Sumoylation | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K489 | Sumoylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| T501 | Phosphorylation | Uniprot |
研究背景
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
Sumoylated.
Nucleus speckle. Nucleus>PML body. Nucleus.
Note: Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity).
Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
Binds SUMO1 and UBE2I. Interacts with AXIN1, JUN, MDM2, PARK7, TP53 and TP73 isoform alpha, but not TP73 isoform beta. Interacts with STAT4 following IL12 and IFN-alpha stimulation of T-cells. Interacts also with GTF2I, GTF2IRD1, IKFZ1, DAB2 and MSX2, as well as with several steroid receptors, including ESR1, ESR2, NR3C1, PGR, AR, and with NCOA2 (By similarity). Sumoylation of a target protein seems to enhance the interaction. Binds to sumoylated ELK1. Binds DNA, such as CDKN1A promoter, in a sequence-specific manner. Interacts with PLAG1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. PIAS2-beta interacts with IFIH1/MDA5. Isoform PIAS2-alpha interacts with PML (isoform PML-12).
The LXXLL motif is a transcriptional coregulator signature.
Belongs to the PIAS family.
研究領域
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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