RBPJ Antibody - #DF7453
(1)
(5)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7453, RRID:AB_2839390.
折りたたみ／展開
AI843960; AOS3; CBF 1; CBF-1; CBF1; csl; IGKJRB; IGKJRB1; J kappa recombination signal binding protein; J kappa-recombination signal-binding protein; KBF2; NY REN 30 antigen; RBP J; RBP J kappa; RBP JK; RBP-J; RBP-J kappa; RBP-JK; Rbpj; RBPJK; RBPSUH; recombination signal binding protein for immunoglobulin kappa J region; Recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; SUH; SUH_HUMAN;
免疫原
- Q06330 SUH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDHTEGSPAEEPPAHAPSPGKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNEKRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDSDKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYLHVEGGNFHASSQQWGAFFIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTALLDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEPNKEMINDGASWTIISTDKAEYTFYEGMGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFREGWRWVRQPVQVPVTLVRNDGIIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPPNESNTNSEGSYTNASTNSTSVTSSTATVVS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q06330 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| K266 | Acetylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| T339 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
研究背景
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).
Nucleus. Cytoplasm.
Note: Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.
Interacts with activated NOTCH1, NOTCH2 or NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with Epstein-Barr virus EBNA2, EBNA3, EBNA4 and EBNA6. Interacts with RITA1/C12orf52, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway. Interacts with SNW1. Interacts with CHCHD2 and CXXC5. Interacts with BEND6 (via BEN domain). Interacts with NKAPL (By similarity). Interacts with ZMIZ1. Interacts with RBM15 (By similarity).
Belongs to the Su(H) family.
研究領域
· Environmental Information Processing > Signal transduction > Notch signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
参考文献
Application: WB Species: Rat Sample: spinal cord tissues
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