PSMB4 Antibody - #DF7465
| 製品: | PSMB4 Antibody |
| カタログ: | DF7465 |
| タンパク質の説明: | Rabbit polyclonal antibody to PSMB4 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 29kDa; 29kD(Calculated). |
| ユニプロット: | P28070 |
| RRID: | AB_2839402 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7465, RRID:AB_2839402.
折りたたみ／展開
26 kDa prosomal protein; HN3; HsBPROS26; HSN3; Macropain beta chain; Multicatalytic endopeptidase complex beta chain; PROS-26; PROS26; Proteasome (prosome macropain) subunit beta type 4; Proteasome beta 4 subunit; Proteasome beta chain; Proteasome chain 3; Proteasome subunit beta type 4; Proteasome subunit beta type-4; Proteasome subunit HsN3; PSB4_HUMAN; PSMB 4; PSMB4;
免疫原
- P28070 PSB4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWDIAHMISGFE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P28070 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| Y22 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| Y75 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| Y102 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| K109 | Sumoylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| Y189 | Phosphorylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot |
研究背景
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Cytoplasm. Nucleus.
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Interacts with PRPF19.
(Microbial infection) Interacts with HTLV-1 Tax protein.
(Microbial infection) Interacts with HIV-1 Nef and Tat proteins.
Belongs to the peptidase T1B family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
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