beta-Tubulin Antibody | Affinity Biosciences

製品:	beta-Tubulin Antibody
カタログ:	T0023
タンパク質の説明:	Mouse monoclonal antibody to beta-Tubulin
アプリケーション:	WB IHC IF/ICC ELISA
反応性:	Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
分子量:	55KD; 50kD(Calculated).
ユニプロット:	P07437
RRID:	AB_2813772

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サイズ	価格	在庫状況
50ul	$150	在庫あり
100ul	$250	在庫あり
200ul	$350	在庫あり
1ml	$1200	在庫あり

プロトコル

製品説明

ソース:

Mouse

アプリケーション:

WB 1:10000-1:500000, IHC 1:50-1:200, IF/ICC: 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat,Sheep,Rabbit,Dog,Monkey,Hamster,Chicken

クローナリティ:

Monoclonal [AFfirm039]

特異性:

beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.

RRID:

AB_2813772
引用形式: Affinity Biosciences Cat# T0023, RRID:AB_2813772.

コンジュゲート:

Unconjugated.

精製:

Affinity-chromatography.

保存:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

TUBB3, CDCBM, Beta III Tubulin, Class III beta-tubulin, TUBB4, Tubulin, beta 3, Tubulin beta-III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin, beta 3 class III, CFEOM3A

免疫原

免疫原:

Full-length beta-tubulin protein of human.

Uniprot:

P07437(TBB5_HUMAN) >>Visit HPA database.

遺伝子(ID):

TUBB(203068)

発現特異性:

P07437 TBB5_HUMAN:

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

タンパク質の説明:

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.

タンパク質配列:

P07437 TBB5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA

PTMs - P07437 基板として

P07437

Site	PTM Type	Enzyme	Source
R2	Methylation		Uniprot
C12	S-Nitrosylation		Uniprot
K19	Acetylation		Uniprot
K19	Methylation		Uniprot
K19	Ubiquitination		Uniprot
S25	Phosphorylation		Uniprot
T33	Phosphorylation		Uniprot
T35	Phosphorylation		Uniprot
Y36	Phosphorylation		Uniprot
S40	Phosphorylation		Uniprot
R46	Methylation		Uniprot
S48	Phosphorylation		Uniprot
Y50	Phosphorylation		Uniprot
Y51	Phosphorylation		Uniprot
T55	Phosphorylation		Uniprot
K58	Acetylation		Uniprot
K58	Methylation		Uniprot
K58	Sumoylation		Uniprot
K58	Ubiquitination		Uniprot
Y59	Phosphorylation		Uniprot
T72	Phosphorylation		Uniprot
S75	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
S95	Phosphorylation		Uniprot
K103	Acetylation		Uniprot
K103	Sumoylation		Uniprot
K103	Ubiquitination		Uniprot
Y106	Phosphorylation		Uniprot
T107	Phosphorylation		Uniprot
S115	Phosphorylation		Uniprot
K122	Ubiquitination		Uniprot
S126	Phosphorylation		Uniprot
T136	Phosphorylation		Uniprot
S138	Phosphorylation		Uniprot
T143	Phosphorylation		Uniprot
S145	Phosphorylation		Uniprot
T149	Phosphorylation		Uniprot
S153	Phosphorylation		Uniprot
K154	Ubiquitination		Uniprot
Y159	Phosphorylation		Uniprot
R162	Methylation		Uniprot
T166	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
S172	Phosphorylation		Uniprot
K174	Ubiquitination		Uniprot
Y183	Phosphorylation		Uniprot
T196	Phosphorylation		Uniprot
T199	Phosphorylation		Uniprot
Y200	Phosphorylation		Uniprot
Y208	Phosphorylation		Uniprot
K216	Ubiquitination		Uniprot
T218	Phosphorylation		Uniprot
T219	Phosphorylation		Uniprot
T221	Phosphorylation		Uniprot
Y222	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
C239	S-Nitrosylation		Uniprot
K252	Sumoylation		Uniprot
K252	Ubiquitination		Uniprot
T274	Phosphorylation		Uniprot
S275	Phosphorylation	Q5TCY1 (TTBK1)	Uniprot
R276	Methylation		Uniprot
S278	Phosphorylation		Uniprot
Y281	Phosphorylation		Uniprot
T285	Phosphorylation		Uniprot
T290	Phosphorylation		Uniprot
K297	Ubiquitination		Uniprot
C303	S-Nitrosylation		Uniprot
Y310	Phosphorylation		Uniprot
T312	Phosphorylation		Uniprot
R318	Methylation		Uniprot
S322	Phosphorylation		Uniprot
K324	Acetylation		Uniprot
K324	Sumoylation		Uniprot
K324	Ubiquitination		Uniprot
K336	Acetylation		Uniprot
K336	Ubiquitination		Uniprot
S338	Phosphorylation		Uniprot
S339	Phosphorylation		Uniprot
Y340	Phosphorylation		Uniprot
K350	Sumoylation		Uniprot
K350	Ubiquitination		Uniprot
T351	Phosphorylation		Uniprot
K362	Ubiquitination		Uniprot
T366	Phosphorylation		Uniprot
S371	Phosphorylation		Uniprot
K379	Acetylation		Uniprot
K379	Ubiquitination		Uniprot
S382	Phosphorylation		Uniprot
T386	Phosphorylation		Uniprot
K392	Ubiquitination		Uniprot
T399	Phosphorylation		Uniprot
Y422	Phosphorylation		Uniprot
Y425	Phosphorylation		Uniprot
T429	Phosphorylation		Uniprot

研究背景

P07437_TBB5_HUMAN

機能:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

PTMs:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

細胞の位置付け:

Cytoplasm>Cytoskeleton.

組織特異性:

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

サブユニット構造:

Heterodimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with PIFO. Interacts with DIAPH1. Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity).

タンパク質ファミリー:

The highly acidic C-terminal region may bind cations such as calcium.

Belongs to the tubulin family.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

参考文献

1). The clinical antiprotozoal drug nitazoxanide and its metabolite tizoxanide extend Caenorhabditis elegans lifespan and healthspan. Acta pharmaceutica Sinica. B, 2024 (PubMed: 39027239) [IF=14.5]

2). ALKBH5 regulates cardiomyocyte proliferation and heart regeneration by demethylating the mRNA of YTHDF1. Theranostics, 2021 (PubMed: 33456585) [IF=12.4]

Application: WB Species: mouse Sample: cardiomyocytes

Figure 8.| ALKBH5 upregulates the mRNA stability of YTHDF1 in turn promotes the translation of YAP. (A) m6A-meRIP-qPCR of YTHDF1 in CTL or ALKBH5 OE P1 CMs (***P < 0.001, n = 4). (B) RT-qPCR analysis of YTHDF1 in cultured P1 cardiomyocytes transfected with control or ALKBH5 OE plasmid (**P < 0.01, n = 4). (C) RT-qPCR analysis of YTHDF1 in cultured P1 cardiomyocytes transfected with control siRNA or ALKBH5 siRNAs (*P < 0.05, n = 4). (D) Western blot analysis of ALKBH5 and YTHDF1 in cultured P1 cardiomyocytes transfected with control or ALKBH5 OE plasmid.

3). Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway. International Journal of Biological Sciences, 2023 (PubMed: 33613114) [IF=9.2]

Application: WB Species: Human Sample: A2780 and OVCAR3 cells

Figure 2 CA125 downregulates DKK1 expression in ovarian cancer cells. qPCR (A-B), ELISA (C-D), and Western blot (E-F) analysis of DKK1 mRNA levels in A2780 and OVCAR3 cells treated with CA125 at 0, 0.2. and 0.4 µg/mL for 48 h. The results represent the mean ± SD. *p<0.05, **p<0.01, ****p<0.0001.

4). EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis. Cell Death & Disease, 2022 (PubMed: 36344490) [IF=9.0]

5). Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. CELL PROLIFERATION, 2022 (PubMed: 35411569) [IF=8.5]

Application: WB Species: rat Sample: Dental pulp fibroblasts

FIGURE 1 |Effects of combined administration of RvE1 and LXA4 on pro-inflammatory factor expression.(C) NLRP3, (D) caspase-1, (E) IL-1β and (F) IL-18 mRNA levels on LPS-induced DPFs detected by qPCR and (G) their protein levels tested by western blotting (normalized to that of β-tubulin).

6). Harmine is an effective therapeutic small molecule for the treatment of cardiac hypertrophy. Acta Pharmacologica Sinica, 2021 (PubMed: 33785860) [IF=8.2]

Application: WB Species: Rat Sample: cardiac tissues

Fig. 8: Harmine attenuates inflammatory response and NF-κB activation both in vivo and vitro. a qPCR analyses of inflammatory-related genes mRNA levels in rat cardiac tissues (normalized to 18S expression). b Representative Western blot analysis of phosphorylated NF-κB p65 and NF-κB p65 protein expression in the heart tissues of WKYs and SHRs after 3-month treatment. c–e Quantification of phosphorylated NF-κB p65 and NF-κB p65 protein expression normalized to β-Tubulin, and phosphorylated NF-κB p65/NF-κB p65 ratio by densitometric analysis. f Representative images of hESC-derived cardiomyocytes stained with cTnT (green) antibody. The hESC-derived cardiomyocytes were treated with LPS (100 ng/mL) for 3 days or were pre-treated with harmine (10 μM) for 3 h and then co-treated with LPS (100 ng/mL) for 3 days (n > 200 per group). Scale bars, 100 μm. g Quantification of cell size changes in each group. h-i Representative immunoblots of phosphorylated NF-κB p65 (p-NF-κB p65) and NF-κB p65 in hESC-derived cardiomyocytes following treatment of LPS (10 μg/mL) for 3 h, or TNF-α (25;ng/mL) for 1 h and 3 h (upper), and quantification of relative protein expression by densitometry (bottom). In all harmine-treated group, hESC-derived cardiomyocytes were pre-treated with harmine (25 μM) for 0.5 h before LPS or TNF-α addition. β-Actin was used as loading control. Data are expressed as mean ± SEM. *P

7). Methyl ferulic acid ameliorates alcohol-induced hepatic insulin resistance via miR-378b-mediated activation of PI3K-AKT pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 34844105) [IF=7.5]

8). MagT1 regulated the odontogenic differentiation of BMMSCs induced byTGC-CM via ERK signaling pathway. Stem Cell Research & Therapy, 2019 (PubMed: 30704530) [IF=7.5]

Application: WB Species: rat Sample: BMMSCs

Fig. 5 | MagT1 was involved in the odontogenic differentiation of BMMSCs.aImmunocytochemistry showed protein expression of MagT1 in BMMSCs. b The protein expression of MagT1 was increased in BMMSCs during odontogenic differentiation. c MagT1-shRNA lentivirus inhibited the mRNA and protein expression of MagT1 in BMMSCs. d The protein levels of ALP, DSP, and DMP-1 were decreased in MagT1-shRNA BMMSCs during odontogenic differentiation after 14d. e In accordance, the extent of mineralization nodules was also significantly decreased.Scale bar = 100 μm

9). Human umbilical cord mesenchymal stem cells-derived exosomes for treating traumatic pancreatitis in rats. Stem Cell Research & Therapy, 2022 (PubMed: 35619158) [IF=7.5]

10). Asiatic acid alleviates cisplatin-induced renal fibrosis in tumor-bearing mice by improving the TFEB-mediated autophagy-lysosome pathway. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37413899) [IF=7.5]

Application: WB Species: Human Sample: HK-2 cells

Fig. 1. AA treatment suppressed renal fibrosis induced by long-term cisplatin injection in nude mice suffering from tumors. (A) Representative images of Sirius red staining and the expression of α-SMA, Collagen I, and Fibronectin in the kidneys of tumor-bearing mice. (B-F) Western blotting and quantitative analysis of the renal expression of Collagen I, Collagen IV, α-SMA, TGF-β1 in tumor-bearing mice. (G-M) Western blotting and quantitative analysis of the expression of Fibronectin, Collagen I, Collagen IV, α-SMA, and TGF-β1 in HK-2 cells. (N-L) RT-PCR detected the renal expression of CTGF and TGF-β1. Each bar represents the mean±SEM from at least three independent experiments. Scale bars= 50 µm. Versus control * P

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beta-Tubulin Antibody - #T0023

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関連ダウンロード

MSDS

説明書

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