MCF2L Antibody - #DF2202
| 製品: | MCF2L Antibody |
| カタログ: | DF2202 |
| タンパク質の説明: | Rabbit polyclonal antibody to MCF2L |
| アプリケーション: | WB IHC |
| 反応性: | Human, Rat |
| 分子量: | 128kDa; 128kD(Calculated). |
| ユニプロット: | O15068 |
| RRID: | AB_2839433 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2202, RRID:AB_2839433.
折りたたみ／展開
ARHGEF 14; DBL''s big sister; DBS; FLJ12122; Guanine nucleotide exchange factor DBS; KIAA0362; MCF 2L; MCF.2 cell line derived transforming sequence like; MCF.2 transforming sequence-like; MCF2 like protein; MCF2 transforming sequence like protein; MCF2-transforming sequence-like protein; Mcf2l; MCF2L_HUMAN; OST; OST oncogene;
免疫原
- O15068 MCF2L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFDCWRFILCKRPGSNSYSSPQRPNEAKKEETDHQIDVSDVIRLVQDTPEATAMATDEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTSHSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSGKAHVPRAHP
PTMs - O15068 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T96 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| S578 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| K608 | Acetylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| Y784 | Phosphorylation | Uniprot | |
| S785 | Phosphorylation | Uniprot | |
| S893 | Phosphorylation | Uniprot | |
| T902 | Phosphorylation | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| T972 | Phosphorylation | Uniprot | |
| S975 | Phosphorylation | Uniprot | |
| T992 | Phosphorylation | Uniprot | |
| Y1000 | Phosphorylation | Uniprot | |
| T1007 | Phosphorylation | Uniprot | |
| S1033 | Phosphorylation | Uniprot | |
| S1034 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42.
Does not catalyze guanine nucleotide exchange on CDC42.
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Cytoplasm. Endomembrane system.
Note: Interaction with membranes enriched in phosphoinositides is mediated by the CRAL-TRIO domain.
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Interacts with GTP-bound RAC1 (By similarity). Interacts with CDC42. Interacts with RHOA. Interacts with CCPG1, which results in specific inhibition of its exchange activity toward RHOA, but does not affect its activity on CDC42 (By similarity).
The CRAL-TRIO domain mediates interaction with various inositol phospholipids, such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P).
The DH domain is involved in interaction with CCPG1.
Belongs to the MCF2 family.
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