UNC5C Antibody - #DF2214

(1)  
製品: UNC5C Antibody
カタログ: DF2214
タンパク質の説明: Rabbit polyclonal antibody to UNC5C
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 66 kDa; 103kD(Calculated).
ユニプロット: O95185
RRID: AB_2839445

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
クローナリティ:
Polyclonal
特異性:
UNC5C Antibody detects endogenous levels of total UNC5C.
RRID:
AB_2839445
引用形式: Affinity Biosciences Cat# DF2214, RRID:AB_2839445.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

homolog of C. elegans transmembrane receptor Unc5; Netrin receptor UNC5C; Protein unc 5 homolog C; Protein unc-5 homolog 3; Protein unc-5 homolog C; Unc 5 homolog 3; Unc 5 homolog C; Unc5 (C.elegans homolog) c; Unc5c; UNC5C_HUMAN; UNC5H3;

免疫原

免疫原:
Uniprot:

O95185(UNC5C_HUMAN) >>Visit HPA database.

遺伝子(ID):
UNC5C(8633)
発現特異性:
O95185 UNC5C_HUMAN:

Mainly expressed in brain (PubMed:9782087). Expressed in temporal lobe cortical neurons and in neurons of the hippocampal pyramidal layer (PubMed:25419706). Also expressed in kidney (PubMed:9782087). Not expressed in developing or adult lung (PubMed:9782087).

タンパク質の説明:
Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
タンパク質配列:
MRKGLRATAARCGLGLGYLLQMLVLPALALLSASGTGSAAQDDDFFHELPETFPSDPPEPLPHFLIEPEEAYIVKNKPVNLYCKASPATQIYFKCNSEWVHQKDHIVDERVDETSGLIVREVSIEISRQQVEELFGPEDYWCQCVAWSSAGTTKSRKAYVRIAYLRKTFEQEPLGKEVSLEQEVLLQCRPPEGIPVAEVEWLKNEDIIDPVEDRNFYITIDHNLIIKQARLSDTANYTCVAKNIVAKRKSTTATVIVYVNGGWSTWTEWSVCNSRCGRGYQKRTRTCTNPAPLNGGAFCEGQSVQKIACTTLCPVDGRWTPWSKWSTCGTECTHWRRRECTAPAPKNGGKDCDGLVLQSKNCTDGLCMQTAPDSDDVALYVGIVIAVIVCLAISVVVALFVYRKNHRDFESDIIDSSALNGGFQPVNIKAARQDLLAVPPDLTSAAAMYRGPVYALHDVSDKIPMTNSPILDPLPNLKIKVYNTSGAVTPQDDLSEFTSKLSPQMTQSLLENEALSLKNQSLARQTDPSCTAFGSFNSLGGHLIVPNSGVSLLIPAGAIPQGRVYEMYVTVHRKETMRPPMDDSQTLLTPVVSCGPPGALLTRPVVLTMHHCADPNTEDWKILLKNQAAQGQWEDVVVVGEENFTTPCYIQLDAEACHILTENLSTYALVGHSTTKAAAKRLKLAIFGPLCCSSLEYSIRVYCLDDTQDALKEILHLERQMGGQLLEEPKALHFKGSTHNLRLSIHDIAHSLWKSKLLAKYQEIPFYHVWSGSQRNLHCTFTLERFSLNTVELVCKLCVRQVEGEGQIFQLNCTVSEEPTGIDLPLLDPANTITTVTGPSAFSIPLPIRQKLCSSLDAPQTRGHDWRMLAHKLNLDRYLNYFATKSSPTGVILDLWEAQNFPDGNLSMLAAVLEEMGRHETVVSLAAEGQY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Zebrafish
82
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95185 基板として

Site PTM Type Enzyme
S123 Phosphorylation
S127 Phosphorylation
T310 Phosphorylation
T311 Phosphorylation
Y449 Phosphorylation
Y454 Phosphorylation
S460 Phosphorylation
T484 Phosphorylation
T506 Phosphorylation
S516 Phosphorylation
S521 Phosphorylation
Y568 Phosphorylation
Y649 Phosphorylation
Y667 Phosphorylation
T674 Phosphorylation
T832 Phosphorylation
S843 Phosphorylation
S854 Phosphorylation
T861 Phosphorylation
Y878 Phosphorylation
Y881 Phosphorylation

研究背景

機能:

Receptor for netrin required for axon guidance (By similarity). Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding (By similarity). NTN1/Netrin-1 binding might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Axon repulsion in growth cones may also be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Might also collaborate with DSCAM in NTN1-mediated axon repulsion independently of DCC (By similarity). Also involved in corticospinal tract axon guidance independently of DCC (By similarity). Involved in dorsal root ganglion axon projection towards the spinal cord. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (By similarity).

PTMs:

Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.

Phosphorylated on different cytoplasmic tyrosine residues. Phosphorylation of Tyr-568 leads to an interaction with PTPN11 phosphatase, suggesting that its activity is regulated by phosphorylation/dephosphorylation. Tyrosine phosphorylation is netrin-dependent.

細胞の位置付け:

Cell membrane>Single-pass type I membrane protein. Cell surface. Cell junction>Synapse>Synaptosome. Cell projection>Axon. Cell projection>Dendrite. Cell projection>Growth cone. Cell projection>Lamellipodium. Cell projection>Filopodium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in brain. Expressed in temporal lobe cortical neurons and in neurons of the hippocampal pyramidal layer. Also expressed in kidney. Not expressed in developing or adult lung.

サブユニット構造:

Interacts with DCC (via cytoplasmic domain) (By similarity). Interacts (tyrosine phosphorylated form) with PTPN11 (By similarity). Interacts (via extracellular domain) with FLRT3 (via extracellular domain) (By similarity). Interacts (via Ig-like C2-type domain) with DSCAM (via extracellular domain). Interacts (via death domain) with DAPK1. Interacts (via cytoplasmic domain) with TUBB3; this interaction is decreased by NTN1/Netrin-1.

タンパク質ファミリー:

Belongs to the unc-5 family.

研究領域

· Organismal Systems > Development > Axon guidance.   (View pathway)

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