SLC12A2 Antibody - #DF2245
(3)
(7)
| 製品: | SLC12A2 Antibody |
| カタログ: | DF2245 |
| タンパク質の説明: | Rabbit polyclonal antibody to SLC12A2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 130 kDa; 131kD(Calculated). |
| ユニプロット: | P55011 |
| RRID: | AB_2839476 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(85%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
SLC12A2 Antibody detects endogenous levels of total SLC12A2.
RRID:
AB_2839476
引用形式: Affinity Biosciences Cat# DF2245, RRID:AB_2839476.
引用形式: Affinity Biosciences Cat# DF2245, RRID:AB_2839476.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; mBSC2; MGC104233; NKCC1; PPP1R141; Protein phosphatase 1, regulatory subunit 141; S12A2_HUMAN; Slc12a2; sodium-potassium-chloride cotransporter 1; Solute carrier family 12 (sodium/potassium/chloride transporter), member 2; solute carrier family 12 (sodium/potassium/chloride transporters) member 2; Solute carrier family 12 member 2; sy-ns;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質の説明:
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume
タンパク質配列:
- P55011 S12A2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPEDAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAAAAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNHQSVLTFYS
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
92
Chicken
85
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P55011 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | P54646 (PRKAA2) , Q13131 (PRKAA1) | Uniprot |
| S79 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| T203 | Phosphorylation | Q9UEW8 (STK39) , O95747 (OXSR1) | Uniprot |
| T207 | Phosphorylation | Q9UEW8 (STK39) , O95747 (OXSR1) | Uniprot |
| Y208 | Phosphorylation | Uniprot | |
| T212 | Phosphorylation | Q9BYP7 (WNK3) , O95747 (OXSR1) , Q9UEW8 (STK39) | Uniprot |
| T217 | Phosphorylation | Q9BYP7 (WNK3) , O95747 (OXSR1) | Uniprot |
| Y227 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | O95747 (OXSR1) | Uniprot |
| K237 | Ubiquitination | Uniprot | |
| S242 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| K252 | Ubiquitination | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| T266 | Phosphorylation | Uniprot | |
| T268 | Phosphorylation | Uniprot | |
| Y275 | Phosphorylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| K833 | Methylation | Uniprot | |
| K833 | Ubiquitination | Uniprot | |
| S836 | Phosphorylation | Uniprot | |
| K841 | Ubiquitination | Uniprot | |
| Y842 | Phosphorylation | Uniprot | |
| Y855 | Phosphorylation | Uniprot | |
| K923 | Ubiquitination | Uniprot | |
| S940 | Phosphorylation | Uniprot | |
| K943 | Ubiquitination | Uniprot | |
| S944 | Phosphorylation | Uniprot | |
| T947 | Phosphorylation | Uniprot | |
| K948 | Ubiquitination | Uniprot | |
| S953 | Phosphorylation | Uniprot | |
| Y956 | Phosphorylation | Uniprot | |
| S957 | Phosphorylation | Uniprot | |
| K958 | Ubiquitination | Uniprot | |
| K959 | Ubiquitination | Uniprot | |
| K966 | Ubiquitination | Uniprot | |
| K971 | Ubiquitination | Uniprot | |
| K976 | Ubiquitination | Uniprot | |
| K983 | Ubiquitination | Uniprot | |
| T984 | Phosphorylation | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| S994 | Phosphorylation | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| K1007 | Ubiquitination | Uniprot | |
| S1012 | Phosphorylation | Uniprot | |
| K1017 | Ubiquitination | Uniprot | |
| K1061 | Ubiquitination | Uniprot | |
| T1074 | Phosphorylation | Uniprot | |
| K1078 | Ubiquitination | Uniprot | |
| K1095 | Ubiquitination | Uniprot | |
| K1097 | Ubiquitination | Uniprot | |
| K1098 | Sumoylation | Uniprot | |
| K1098 | Ubiquitination | Uniprot | |
| K1118 | Ubiquitination | Uniprot | |
| K1125 | Ubiquitination | Uniprot | |
| K1127 | Ubiquitination | Uniprot | |
| K1143 | Ubiquitination | Uniprot | |
| Y1211 | Phosphorylation | Uniprot | |
| S1212 | Phosphorylation | Uniprot |
研究背景
機能:
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
細胞の位置付け:
Membrane>Multi-pass membrane protein.
組織特異性:
Expressed in many tissues.
タンパク質ファミリー:
Belongs to the SLC12A transporter family.
研究領域
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
参考文献
1). Activated WNK3 induced by intracerebral hemorrhage deteriorates brain injury maybe via WNK3/SPAK/NKCC1 pathway. EXPERIMENTAL NEUROLOGY, 2020
(PubMed: 32589890)
[IF=5.3]
Application: WB Species: Rat Sample: brain tissue
Fig. 6. Effects of WNK3 on the levels of p-SPAK and p-NKCC1 in rat brain tissue after ICH. (A) Levels of WNK3 protein were significantly changed in various treated
ICH groups. (B) The levels of p-SPAK protein were significantly changed in various groups. (C) The levels of p-NKCC1 protein were significantly changed in various
groups. Data are presented as the mean ± SD. **P < .01 vs. Sham group; ns, no significant differences vs. ICH group; ##P < .01 vs. ICH + si-NC group; &&
P < .01 vs. ICH + Vector group; n = 6.
2). Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model. Nutrition & Metabolism, 2021
(PubMed: 33691721)
[IF=4.5]
Application: WB Species: Human Sample: NAFLD
Fig. 4
Identification of the changed expression levels of proteins in NAFLD with and without Kaili Sour Soup treatment. a and b Western blot analysis of the protein expression in NAFLD with and without Kaili Sour Soup treatment. β-Actin served as a reference control
3). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023
(PubMed: 37212774)
[IF=2.1]
Application: WB Species: Human Sample:
Fig. 3. Aquaporin 5 (AQP5) and Na-K-Cl cotransporter 1 (NKCC1) protein expression levels in sweat gland cells extracted from patients with primary palmar hyperhidrosis treated with blank control or transfected with overexpression negative control (OE NC) or integrin β6 (ITGB6) OE. Western blot images (A) and quantitative analyses (B) (ITGB6 OE compared to control, AQP5: Tukey’s honest significant difference (HSD), p = 0.055; NKCC1: Tukey’s HSD, p = 0.190). The data are presented as mean ± 95% confidence interval (95% CI)
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