TAF7 Antibody - #DF2253
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2253, RRID:AB_2839482.
折りたたみ／展開
RNA polymerase II TBP-associated factor subunit F; TAF 7; TAF(II)55; TAF2F; TAF7; TAF7 RNA polymerase II; TAF7 RNA polymerase II TATA box binding protein (TBP) associated factor 55 kDa; TAF7_HUMAN; TAFII 55; TAFII-55; TAFII55; TATA box binding protein (TBP) associated factor RNA polymerase II F 55kD; TATA box binding protein associated factor 2F; TBP associated factor F; Transcription factor IID subunit TAFII55; Transcription initiation factor TFIID 55 kDa subunit; Transcription initiation factor TFIID subunit 7;
免疫原
- Q15545 TAF7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKSKDDAPHELESQFILRLPPEYASTVRRAVQSGHVNLKDRLTIELHPDGRHGIVRVDRVPLASKLVDLPCVMESLKTIDKKTFYKTADICQMLVSTVDGDLYPPVEEPVASTDPKASKKKDKDKEKKFIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQGHDSLEHDELREIFNDLSSSSEDEDETQHQDEEDINIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMGIQKQIDNMKGKLQETQDRAKRQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15545 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Methylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K129 | Sumoylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | P21675 (TAF1) | Uniprot |
| T274 | Phosphorylation | Uniprot | |
| K291 | Acetylation | Uniprot |
研究背景
Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
Phosphorylated by CIITA. Phosphorylation at Ser-264 by TAF1 in early G1 phase disrupts binding to TAF1.
Nucleus.
Ubiquitous.
TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Interacts with TAF1; the interaction is direct. Interacts with TAFII250, TAFII100, TAFII28, TAFII20, and TAFII18, but not with TAFII30 or TBP. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Binds to CIITA and TAF1 and inhibits their acetyltransferase activity, thereby repressing activated transcription and behaving as a repressor at these promoters.
The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which methylates Lys-5 in vitro.
Belongs to the TAF7 family.
研究領域
· Genetic Information Processing > Transcription > Basal transcription factors.
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