USP1 Antibody - #DF2257
製品: | USP1 Antibody |
カタログ: | DF2257 |
タンパク質の説明: | Rabbit polyclonal antibody to USP1 |
アプリケーション: | WB IHC |
反応性: | Human |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
分子量: | 88 kDa; 88kD(Calculated). |
ユニプロット: | O94782 |
RRID: | AB_2839486 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2257, RRID:AB_2839486.
折りたたみ/展開
Deubiquitinating enzyme 1; hUBP; Ubiquitin carboxyl terminal hydrolase 1; Ubiquitin carboxyl-terminal hydrolase 1; Ubiquitin specific peptidase 1; Ubiquitin specific protease 1; Ubiquitin thioesterase 1; Ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP; UBP1_HUMAN; USP 1; Usp1;
免疫原
- O94782 UBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O94782 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S7 | Phosphorylation | Uniprot | |
S13 | Phosphorylation | Uniprot | |
S16 | Phosphorylation | Uniprot | |
T33 | Phosphorylation | Uniprot | |
S42 | Phosphorylation | Uniprot | |
K48 | Ubiquitination | Uniprot | |
S67 | Phosphorylation | Uniprot | |
K73 | Ubiquitination | Uniprot | |
K110 | Ubiquitination | Uniprot | |
K124 | Sumoylation | Uniprot | |
K130 | Acetylation | Uniprot | |
K224 | Ubiquitination | Uniprot | |
K232 | Sumoylation | Uniprot | |
K232 | Ubiquitination | Uniprot | |
S248 | Phosphorylation | Uniprot | |
S273 | Phosphorylation | Uniprot | |
K283 | Ubiquitination | Uniprot | |
K285 | Ubiquitination | Uniprot | |
T307 | Phosphorylation | Uniprot | |
S308 | Phosphorylation | Uniprot | |
T310 | Phosphorylation | Uniprot | |
S313 | Phosphorylation | P06493 (CDK1) | Uniprot |
S323 | Phosphorylation | Uniprot | |
S327 | Phosphorylation | Uniprot | |
K343 | Ubiquitination | Uniprot | |
S350 | Phosphorylation | Uniprot | |
K360 | Ubiquitination | Uniprot | |
K368 | Ubiquitination | Uniprot | |
S398 | Phosphorylation | Uniprot | |
S475 | Phosphorylation | Uniprot | |
K483 | Ubiquitination | Uniprot | |
K503 | Ubiquitination | Uniprot | |
K523 | Ubiquitination | Uniprot | |
T529 | Phosphorylation | Uniprot | |
K610 | Ubiquitination | Uniprot | |
K623 | Ubiquitination | Uniprot | |
K656 | Ubiquitination | Uniprot | |
K675 | Ubiquitination | Uniprot | |
Y678 | Phosphorylation | Uniprot | |
Y681 | Phosphorylation | Uniprot | |
K683 | Ubiquitination | Uniprot | |
K689 | Ubiquitination | Uniprot | |
K716 | Acetylation | Uniprot | |
K716 | Ubiquitination | Uniprot | |
S727 | Phosphorylation | Uniprot | |
K732 | Ubiquitination | Uniprot | |
K741 | Ubiquitination | Uniprot | |
K746 | Ubiquitination | Uniprot | |
K761 | Ubiquitination | Uniprot | |
S768 | Phosphorylation | Uniprot | |
S770 | Phosphorylation | Uniprot | |
S772 | Phosphorylation | Uniprot | |
T774 | Phosphorylation | Uniprot | |
S775 | Phosphorylation | Uniprot |
研究背景
Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
Ubiquitinated; leading to its subsequent proteasomal degradation.
Nucleus.
Interacts with FANCD2 and PCNA. Interacts with WDR48.
Belongs to the peptidase C19 family.
研究領域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
参考文献
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.