USP28 Antibody - #DF2258

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製品: USP28 Antibody
カタログ: DF2258
タンパク質の説明: Rabbit polyclonal antibody to USP28
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 122 kDa; 122kD(Calculated).
ユニプロット: Q96RU2
RRID: AB_2839487

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 100ul $280 在庫あり
 200ul $350 在庫あり

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
USP28 Antibody detects endogenous levels of total USP28.
RRID:
AB_2839487
引用形式: Affinity Biosciences Cat# DF2258, RRID:AB_2839487.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Deubiquitinating enzyme 28; KIAA1515; Ubiquitin carboxyl terminal hydrolase 28; Ubiquitin carboxyl-terminal hydrolase 28; ubiquitin carboxyl-terminal hydrolase 28 variant 1; Ubiquitin specific peptidase 28; Ubiquitin specific processing protease 28; Ubiquitin specific protease 28; Ubiquitin thioesterase 28; Ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28; UBP28_HUMAN; USP 28; USP28; USP28 protein;

免疫原

免疫原:
Uniprot:

Q96RU2(UBP28_HUMAN) >>Visit HPA database.

遺伝子(ID):
USP28(57646)
タンパク質の説明:
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus
タンパク質配列:
MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAALSEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Horse
88
Bovine
88
Sheep
88
Chicken
57
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RU2 基板として

Site PTM Type Enzyme
K45 Ubiquitination
K64 Ubiquitination
S67 Phosphorylation Q13315 (ATM)
T70 Phosphorylation
K85 Ubiquitination
K90 Ubiquitination
K99 Sumoylation
S113 Phosphorylation
T130 Phosphorylation
S131 Phosphorylation
T134 Phosphorylation
K243 Ubiquitination
S279 Phosphorylation
S375 Phosphorylation
K381 Ubiquitination
K420 Ubiquitination
K425 Ubiquitination
K431 Ubiquitination
Y447 Phosphorylation
S493 Phosphorylation
S495 Phosphorylation Q13315 (ATM)
S503 Phosphorylation
S504 Phosphorylation
K513 Ubiquitination
S520 Phosphorylation
S521 Phosphorylation
K542 Ubiquitination
T543 Phosphorylation
S550 Phosphorylation
Y654 Phosphorylation
S714 Phosphorylation Q13315 (ATM)
S720 Phosphorylation Q13315 (ATM)
T753 Phosphorylation
T788 Phosphorylation
K839 Ubiquitination
K853 Ubiquitination
K897 Ubiquitination
Y901 Phosphorylation
T1048 Phosphorylation
S1053 Phosphorylation
S1059 Phosphorylation

研究背景

機能:

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.

PTMs:

Degraded upon nickel ion level or hypoxia exposure.

Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. Phosphorylated by PRKD1.

細胞の位置付け:

Nucleus>Nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with ZNF304. Interacts with PRKD1. Interacts with TP53BP1. Interacts with isoform 1 of FBXW7; following DNA damage, dissociates from FBXW7 leading to degradation of MYC.

タンパク質ファミリー:

Belongs to the peptidase C19 family. USP28 subfamily.

参考文献

1). circFBXW7 Inhibits Malignant Progression by Sponging miR-197-3p and Encoding a 185-aa Protein in Triple-Negative Breast Cancer. Molecular Therapy-Nucleic Acids, 2019 (PubMed: 31536884) [IF=8.8]

Application: WB    Species: human    Sample: BT549 cells

Figure 6.| FBXW7-185aa, Encoded by circFBXW7, Inhibited the Proliferation and Metastasis of TNBC Cells through Downregulating c-Myc Expression.s. (H)FBXW7-185aa-FLAG-overexpressing BT549 cells were cotransfected with sh-FBXW7 or USP28 vectors. The FBXW7-185aaFLAG, FBXW7, USP28, and c-Myc expression levels were quantified by western blot analysis.

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