VPS4B Antibody - #DF2264
製品: | VPS4B Antibody |
カタログ: | DF2264 |
タンパク質の説明: | Rabbit polyclonal antibody to VPS4B |
アプリケーション: | WB |
反応性: | Human, Mouse, Rat |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
分子量: | 49 kDa; 49kD(Calculated). |
ユニプロット: | O75351 |
RRID: | AB_2839493 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2264, RRID:AB_2839493.
折りたたみ/展開
8030489C12Rik; Cell migration inducing 1; Cell migration-inducing gene 1 protein; MGC116271; MIG1; Protein SKD1; Skd1; SKD1B; Suppressor of K(+) transport growth defect 1; Suppressor of K+ transport defect 1; Vacuolar protein sorting 4 homolog B (S. cerevisiae); Vacuolar protein sorting 4 homolog B; Vacuolar protein sorting 4b; Vacuolar protein sorting-associated protein 4B; Vacuolar protein sorting-associating protein 4B; VPS4 2; VPS42; Vps4b; VPS4B_HUMAN;
免疫原
- O75351 VPS4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75351 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Phosphorylation | Uniprot | |
T4 | Phosphorylation | Uniprot | |
S5 | Phosphorylation | Uniprot | |
K10 | Ubiquitination | Uniprot | |
K17 | Ubiquitination | Uniprot | |
Y40 | Phosphorylation | Uniprot | |
K53 | Acetylation | Uniprot | |
S57 | Phosphorylation | Uniprot | |
T63 | Phosphorylation | Uniprot | |
K88 | Ubiquitination | Uniprot | |
S93 | Phosphorylation | Uniprot | |
S102 | Phosphorylation | Uniprot | |
S108 | Phosphorylation | Uniprot | |
K143 | Ubiquitination | Uniprot | |
K147 | Ubiquitination | Uniprot | |
K155 | Ubiquitination | Uniprot | |
K163 | Acetylation | Uniprot | |
K163 | Ubiquitination | Uniprot | |
T178 | Phosphorylation | Uniprot | |
K180 | Ubiquitination | Uniprot | |
Y182 | Phosphorylation | Uniprot | |
K214 | Ubiquitination | Uniprot | |
K217 | Ubiquitination | Uniprot | |
K227 | Ubiquitination | Uniprot | |
T317 | Phosphorylation | Uniprot | |
S320 | Phosphorylation | Uniprot | |
K332 | Ubiquitination | Uniprot | |
Y336 | Phosphorylation | Uniprot | |
S337 | Phosphorylation | Uniprot | |
S342 | Phosphorylation | Uniprot | |
K355 | Ubiquitination | Uniprot | |
S358 | Phosphorylation | Uniprot | |
T360 | Phosphorylation | Uniprot | |
K363 | Ubiquitination | Uniprot | |
S385 | Phosphorylation | Uniprot | |
S410 | Phosphorylation | Uniprot | |
K422 | Ubiquitination | Uniprot | |
K432 | Ubiquitination | Uniprot | |
K435 | Ubiquitination | Uniprot |
研究背景
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.
Prevacuolar compartment membrane>Peripheral membrane protein. Late endosome membrane>Peripheral membrane protein.
Note: Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
Ubiquitously expressed.
Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. In vitro, associates on the inside of a helical tubular structure formed by a CHMP2A-CHMP3 polymer. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP4B and CHMP6. Interacts with VPS4A; the interaction suggests a heteromeric assembly with VPS4A. Interacts with VTA1.
The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
Belongs to the AAA ATPase family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
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