CCNT2 Antibody - #DF2289
| 製品: | CCNT2 Antibody |
| カタログ: | DF2289 |
| タンパク質の説明: | Rabbit polyclonal antibody to CCNT2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 81kDa; 81kD(Calculated). |
| ユニプロット: | O60583 |
| RRID: | AB_2839515 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2289, RRID:AB_2839515.
折りたたみ／展開
CCNT2; CCNT2_HUMAN; Cyclin T2a; Cyclin T2b; Cyclin-T2; CycT2; FLJ90560; MGC134840; SDS stable vimentin bound DNA fragment HEF42VIM22; Subunit of positive elongation transcription factor b;
免疫原
- O60583 CCNT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASGRGASSRWFFTREQLENTPSRRCGVEADKELSCRQQAANLIQEMGQRLNVSQLTINTAIVYMHRFYMHHSFTKFNKNIISSTALFLAAKVEEQARKLEHVIKVAHACLHPLEPLLDTKCDAYLQQTQELVILETIMLQTLGFEITIEHPHTDVVKCTQLVRASKDLAQTSYFMATNSLHLTTFCLQYKPTVIACVCIHLACKWSNWEIPVSTDGKHWWEYVDPTVTLELLDELTHEFLQILEKTPNRLKKIRNWRANQAARKPKVDGQVSETPLLGSSLVQNSILVDSVTGVPTNPSFQKPSTSAFPAPVPLNSGNISVQDSHTSDNLSMLATGMPSTSYGLSSHQEWPQHQDSARTEQLYSQKQETSLSGSQYNINFQQGPSISLHSGLHHRPDKISDHSSVKQEYTHKAGSSKHHGPISTTPGIIPQKMSLDKYREKRKLETLDLDVRDHYIAAQVEQQHKQGQSQAASSSSVTSPIKMKIPIANTEKYMADKKEKSGSLKLRIPIPPTDKSASKEELKMKIKVSSSERHSSSDEGSGKSKHSSPHISRDHKEKHKEHPSSRHHTSSHKHSHSHSGSSSGGSKHSADGIPPTVLRSPVGLSSDGISSSSSSSRKRLHVNDASHNHHSKMSKSSKSSGSSSSSSSSVKQYISSHNSVFNHPLPPPPPVTYQVGYGHLSTLVKLDKKPVETNGPDANHEYSTSSQHMDYKDTFDMLDSLLSAQGMNM
PTMs - O60583 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K407 | Sumoylation | Uniprot | |
| T426 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K493 | Acetylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| T514 | Phosphorylation | Uniprot | |
| K520 | Acetylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| T597 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S645 | Phosphorylation | Uniprot |
研究背景
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). The activity of this complex is regulated by binding with 7SK snRNA. Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (By similarity). In addition, enhances MYOD1-dependent transcription through interaction with PKN1. Involved in early embryo development (By similarity).
(Microbial infection) Promotes transcriptional activation of early and late herpes simplex virus 1/HHV-1 promoters.
Cytoplasm>Perinuclear region. Nucleus.
Note: Nucleus in differentiating cells.
Ubiquitously expressed.
Interacts with CDK9 to form P-TEFb. Interacts with POLR2A (via the C-terminal domain (CTD)); mediates transcriptional activity. Interacts with HEXIM1; mediates formation of a tripartite complex with KPNA2. Interacts with HEXIM2. Interacts with PKN1; enhances MYOD1-dependent transcription. P-TEFB complex interacts with RB1; promotes phosphorylation of RB1. P-TEFB complex interacts with MYOD1; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity). Interacts with MDFI and MDFIC. Interacts with MON1B; downregulates CCNT2-mediated activation of viral promoters during herpes simplex virus 1/HHV-1 infection.
(Microbial infection) Interacts with HIV-2 and SIV Tat. Does not bind efficiently to the transactivation domain of the HIV-1 Tat.
Belongs to the cyclin family. Cyclin C subfamily.
研究領域
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
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