CENPR Antibody - #DF2317
| 製品: | CENPR Antibody |
| カタログ: | DF2317 |
| タンパク質の説明: | Rabbit polyclonal antibody to CENPR |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Dog |
| 分子量: | 20 kDa; 20kD(Calculated). |
| ユニプロット: | Q13352 |
| RRID: | AB_2839541 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2317, RRID:AB_2839541.
折りたたみ／展開
Beta 3 endonexin; Beta3 endonexin; Beta3 endonexin long form; CENP R; CENPR; CENPR_HUMAN; Centromere protein R; endonexin, Beta-3; HSU37139; Integrin beta 3 binding protein (beta3-endonexin); Integrin beta 3 binding protein; NRIF 3; NRIF3; Nuclear receptor interacting factor 3; TAP 20; TAP20;
免疫原
Widely expressed. Expressed in spleen, thymus, prostate, ovary, small intestine and white blood cells. Highly expressed in testis and colon. Isoform 4 is expressed in platelets, lymphocytes and granulocytes.
- Q13352 CENPR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPVKRSLKLDGLLEENSFDPSKITRKKSVITYSPTTGTCQMSLFASPTSSEEQKHRNGLSNEKRKKLNHPSLTESKESTTKDNDEFMMLLSKVEKLSEEIMEIMQNLSSIQALEGSRELENLIGISCASHFLKREMQKTKELMTKVNKQKLFEKSTGLPHKASRHLDSYEFLKAILN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13352 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| Y32 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S46 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S60 | Phosphorylation | Uniprot | |
| K66 | Sumoylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K92 | Sumoylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| S168 | Phosphorylation | Uniprot |
研究背景
Transcription coregulator that can have both coactivator and corepressor functions. Isoform 1, but not other isoforms, is involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. In contrast, it does not coactivate nuclear receptors for retinoic acid, vitamin D, progesterone receptor, nor glucocorticoid. Acts as a coactivator for estrogen receptor alpha. Acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. May also act as an inhibitor of cyclin A-associated kinase. Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.
Nucleus. Chromosome>Centromere. Chromosome>Centromere>Kinetochore.
Nucleus.
Nucleus. Cytoplasm.
Note: Isoform 3 is predominantly nuclear and weakly cytoplasmic.
Cytoplasm.
Widely expressed. Expressed in spleen, thymus, prostate, ovary, small intestine and white blood cells. Highly expressed in testis and colon. Isoform 4 is expressed in platelets, lymphocytes and granulocytes.
Homodimer; mediated by the coiled coil domain. Isoform 3, but not other isoforms, interacts with the cytoplasmic tail of integrin ITGB3. The relevance of the interaction with ITGB3 is however uncertain, since isoform 3 is mainly nuclear. Interacts with CCNA2 and MTA1. Interacts with NFKB1 NF-kappa-B subunit. Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU.
The DD1 domain (also called RepD1 domain) mediates the corepressor function and is essential in the triggering of apoptosis.
Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, a motif known to be important for the association with nuclear receptors. Such motif, which is required for an efficient association with nuclear receptors, is however not essential.
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL) motif, which is essential for the association with nuclear receptors.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.