CENPU Antibody - #DF2320

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製品: CENPU Antibody
カタログ: DF2320
タンパク質の説明: Rabbit polyclonal antibody to CENPU
アプリケーション: WB IHC
反応性: Human, Mouse
分子量: 48 kDa; 48kD(Calculated).
ユニプロット: Q71F23
RRID: AB_2839544

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
CENPU Antibody detects endogenous levels of total CENPU.
RRID:
AB_2839544
引用形式: Affinity Biosciences Cat# DF2320, RRID:AB_2839544.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CENP U; CENP-50; CENP-U; CENP50; CENPU; CENPU_HUMAN; CENPU50; centromere protein of 50 kDa; Centromere protein U; FLJ23468; HHV8 LNAIP1; ICEN24; Interphase centromere complex protein 24; Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1; KLIP-1; KLIP1; KSHV latent nuclear antigen interacting protein 1; KSHV latent nuclear antigen-interacting protein 1; KSHV LNAIP1; LNAIP 1; LNAIP-1; MLF1-interacting protein; MLF1IP; MLF1IP1; PBIP1; Polo-box-interacting protein 1;

免疫原

免疫原:
Uniprot:

Q71F23(CENPU_HUMAN) >>Visit HPA database.

遺伝子(ID):
CENPU(79682)
発現特異性:
Q71F23 CENPU_HUMAN:

Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.

タンパク質の説明:
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
タンパク質配列:
MAPRGRRRPRPHRSEGARRSKNTLERTHSMKDKAGQKCKPIDVFDFPDNSDVSSIGRLGENEKDEETYETFDPPLHSTAIYADEEEFSKHCGLSLSSTPPGKEAKRSSDTSGNEASEIESVKISAKKPGRKLRPISDDSESIEESDTRRKVKSAEKISTQRHEVIRTTASSELSEKPAESVTSKKTGPLSAQPSVEKENLAIESQSKTQKKGKISHDKRKKSRSKAIGSDTSDIVHIWCPEGMKTSDIKELNIVLPEFEKTHLEHQQRIESKVCKAAIATFYVNVKEQFIKMLKESQMLTNLKRKNAKMISDIEKKRQRMIEVQDELLRLEPQLKQLQTKYDELKERKSSLRNAAYFLSNLKQLYQDYSDVQAQEPNVKETYDSSSLPALLFKARTLLGAESHLRNINHQLEKLLDQG

PTMs - Q71F23 基板として

Site PTM Type Enzyme
S50 Phosphorylation
S53 Phosphorylation
S54 Phosphorylation
Y68 Phosphorylation
S77 Phosphorylation P53350 (PLK1)
T78 Phosphorylation P53350 (PLK1)
Y81 Phosphorylation
S94 Phosphorylation
S96 Phosphorylation
S97 Phosphorylation
T98 Phosphorylation
S107 Phosphorylation
S108 Phosphorylation
T110 Phosphorylation
S111 Phosphorylation
S116 Phosphorylation
S136 Phosphorylation
S139 Phosphorylation
S141 Phosphorylation
T147 Phosphorylation
S171 Phosphorylation
K176 Ubiquitination
K185 Ubiquitination
T186 Phosphorylation
S190 Phosphorylation
S194 Phosphorylation
K197 Ubiquitination
S204 Phosphorylation
S206 Phosphorylation
S229 Phosphorylation
S232 Phosphorylation
K244 Ubiquitination
K249 Ubiquitination
K260 Ubiquitination
K272 Ubiquitination
K291 Acetylation
T300 Phosphorylation
K303 Acetylation
K303 Methylation
K303 Ubiquitination
K308 Methylation
K335 Ubiquitination
K340 Ubiquitination
K379 Ubiquitination
K393 Ubiquitination
K413 Ubiquitination

研究背景

機能:

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.

PTMs:

Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.

細胞の位置付け:

Cytoplasm. Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.

サブユニット構造:

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with MLF1. Interacts with PLK1.

(Microbial infection) Interacts with the N-terminal domain of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen (LNA).

タンパク質ファミリー:

Belongs to the CENP-U/AME1 family.

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