CUL5 Antibody - #DF2323
| 製品: | CUL5 Antibody |
| カタログ: | DF2323 |
| タンパク質の説明: | Rabbit polyclonal antibody to CUL5 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 91 kDa.; 91kD(Calculated). |
| ユニプロット: | Q93034 |
| RRID: | AB_2839547 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2323, RRID:AB_2839547.
折りたたみ／展開
CUL 5; CUL-5; CUL5; CUL5_HUMAN; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); Cullin-5; Cullin5; VACM 1; VACM-1; VACM1; Vasopressin activated calcium mobilizing receptor 1; Vasopressin activated calcium mobilizing receptor; Vasopressin-activated calcium-mobilizing receptor 1; Vasopressin-activated calcium-mobilizing receptor-1;
免疫原
- Q93034 CUL5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q93034 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K286 | Acetylation | Uniprot | |
| T346 | Phosphorylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| Y373 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K447 | Acetylation | Uniprot | |
| K455 | Acetylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| K620 | Ubiquitination | Uniprot | |
| R631 | Methylation | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| T695 | Phosphorylation | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| S730 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y765 | Phosphorylation | Uniprot |
研究背景
Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN).
Component of multiple ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes formed of CUL5, Elongin BC (ELOB and ELOC), RBX2 and a variable SOCS box domain-containing protein as substrate-specific recognition component. Component of the probable ECS(LRRC41) complex with the substrate recognition component LRRC41. Component of the probable ECS(SOCS1) complex with the substrate recognition component SOCS1. Component of the probable ECS(WSB1) complex with the substrate recognition subunit WSB1. Component of the probable ECS(SOCS3) complex with the substrate recognition component SOCS3. Component of the probable ECS(SPSB1) complex with the substrate recognition component SPSB1. Component of the probable ECS(SPSB2) complex with the substrate recognition component SPSB2. Component of the probable ECS(SPSB4) complex with the substrate recognition component SPSB4. Component of the probable ECS(RAB40C) complex with the substrate recognition subunit RAB40C. May also form complexes containing CUL5, elongin BC complex (ELOB and ELOC), RBX1 and ELOA. May also form complexes containing CUL5, Elongin BC (ELOB and ELOC), RBX1 and VHL. Interacts with RNF7/RBX2, LRRC41, SOCS3, SPSB1, SPSB2, SPSB4 and RAB40C. Interacts with ASB1, ASB2, ASB6, ASB7 and ASB12. Interacts (when neddylated) with ARIH2; leading to activate the E3 ligase activity of ARIH1. Interacts with NOS2 in the presence of SPSB1 or SPSB2 or SPSB4. Interacts with ERCC6; the interaction is induced by DNA damaging agents or inhibitors of RNA polymerase II elongation. Interacts with ELOA (via BC-box).
(Microbial infection) Interacts (via the substrate recognition component) with HIV-1 Vif.
(Microbial infection) Interacts (via the substrate recognition component) with human adenovirus 5 proteins E1B-55K and E4-orf6.
(Microbial infection) Interacts with herpes virus 8 protein LANA1; this interaction promotes the degradation of NF-kappa-B component RELA.
(Microbial infection) Interacts with molluscum contagiosum virus protein MC132; this interaction promotes the degradation of NF-kappa-B component RELA.
Belongs to the cullin family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.