JNK2 Antibody - #DF2357
| 製品: | JNK2 Antibody |
| カタログ: | DF2357 |
| タンパク質の説明: | Rabbit polyclonal antibody to JNK2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 54 kDa; 48kD(Calculated). |
| ユニプロット: | P45984 |
| RRID: | AB_2839565 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2357, RRID:AB_2839565.
折りたたみ／展開
c Jun kinase 2; C Jun N terminal kinase 2; c-Jun N-terminal kinase 2; JNK 55; JNK-55; JNK2 alpha; JNK2; JNK2 beta; JNK2A; JNK2alpha; JNK2B; JNK2BETA; Jun kinase; MAP kinase 9; MAPK 9; Mapk9; Mitogen activated protein kinase 9; Mitogen-activated protein kinase 9; MK09_HUMAN; P54a; p54aSAPK; PRKM9; Protein kinase, mitogen-activated, 9; SAPK alpha; SAPK; SAPK1a; Stress activated protein kinase 1a; Stress-activated protein kinase JNK2;
免疫原
A synthesized peptide derived from human MAPK9, corresponding to a region within N-terminal amino acids.
- P45984 MK09_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGCR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P45984 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K56 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| C163 | S-Nitrosylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | O14733 (MAP2K7) | Uniprot |
| Y185 | Phosphorylation | P45985 (MAP2K4) , P07949 (RET) , O14733 (MAP2K7) | Uniprot |
| T188 | Phosphorylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| T386 | Phosphorylation | Uniprot | |
| T404 | Phosphorylation | O14733 (MAP2K7) , P68400 (CSNK2A1) | Uniprot |
| S407 | Phosphorylation | P68400 (CSNK2A1) , O14733 (MAP2K7) | Uniprot |
PTMs - P45984 酵素として
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S396 | Uniprot |
| O43521-2 (BCL2L11) | T56 | Uniprot |
| O43521-1 (BCL2L11) | S59 | Uniprot |
| O43521 (BCL2L11) | S69 | Uniprot |
| O43521-1 (BCL2L11) | S77 | Uniprot |
| O43602-2 (DCX) | T321 | Uniprot |
| O43602-2 (DCX) | T331 | Uniprot |
| O43602-2 (DCX) | S334 | Uniprot |
| O60239-2 (SH3BP5) | S194 | Uniprot |
| O60239-2 (SH3BP5) | S264 | Uniprot |
| O60239 (SH3BP5) | S351 | Uniprot |
| O60239-1 (SH3BP5) | S421 | Uniprot |
| O95140 (MFN2) | S27 | Uniprot |
| P01106 (MYC) | S62 | Uniprot |
| P01106 (MYC) | S71 | Uniprot |
| P04150 (NR3C1) | S226 | Uniprot |
| P04637 (TP53) | S6 | Uniprot |
| P04637 (TP53) | S20 | Uniprot |
| P04637 (TP53) | S37 | Uniprot |
| P04637 (TP53) | T81 | Uniprot |
| P05067-4 (APP) | T668 | Uniprot |
| P05067 (APP) | T743 | Uniprot |
| P05412 (JUN) | S63 | Uniprot |
| P05412 (JUN) | S73 | Uniprot |
| P10636-8 (MAPT) | T181 | Uniprot |
| P10636-8 (MAPT) | S202 | Uniprot |
| P10636-8 (MAPT) | T212 | Uniprot |
| P10636-8 (MAPT) | T217 | Uniprot |
| P10636-8 (MAPT) | T231 | Uniprot |
| P10636 (MAPT) | T498 | Uniprot |
| P10636 (MAPT) | S516 | Uniprot |
| P10636 (MAPT) | S519 | Uniprot |
| P10636 (MAPT) | T529 | Uniprot |
| P10636 (MAPT) | T548 | Uniprot |
| P10636 (MAPT) | S713 | Uniprot |
| P10636 (MAPT) | S721 | Uniprot |
| P10636 (MAPT) | S739 | Uniprot |
| P15036 (ETS2) | T72 | Uniprot |
| P15336 (ATF2) | T69 | Uniprot |
| P15336 (ATF2) | T71 | Uniprot |
| P15336 (ATF2) | S90 | Uniprot |
| P16104 (H2AFX) | S140 | Uniprot |
| P16949 (STMN1) | S25 | Uniprot |
| P16949 (STMN1) | S38 | Uniprot |
| P19419-1 (ELK1) | S383 | Uniprot |
| P19419 (ELK1) | S389 | Uniprot |
| P19793 (RXRA) | S56 | Uniprot |
| P19793 (RXRA) | S70 | Uniprot |
| P19793 (RXRA) | T82 | Uniprot |
| P19793 (RXRA) | S260 | Uniprot |
| P23443 (RPS6KB1) | S434 | Uniprot |
| P29353 (SHC1) | S36 | Uniprot |
| P30307 (CDC25C) | S168 | Uniprot |
| P31645 (SLC6A4) | T616 | Uniprot |
| P31947 (SFN) | S186 | Uniprot |
| P35222 (CTNNB1) | S191 | Uniprot |
| P35222 (CTNNB1) | S605 | Uniprot |
| P35568 (IRS1) | S307 | Uniprot |
| P35568 (IRS1) | S315 | Uniprot |
| P35568 (IRS1) | Y612 | Uniprot |
| P35568 (IRS1) | Y632 | Uniprot |
| P40763 (STAT3) | S727 | Uniprot |
| P41970 (ELK3) | S357 | Uniprot |
| P41970 (ELK3) | S363 | Uniprot |
| P41970 (ELK3) | S396 | Uniprot |
| P41970 (ELK3) | S401 | Uniprot |
| P42224 (STAT1) | S727 | Uniprot |
| P46937 (YAP1) | T119 | Uniprot |
| P46937 (YAP1) | S138 | Uniprot |
| P46937 (YAP1) | T154 | Uniprot |
| P46937 (YAP1) | S367 | Uniprot |
| P49768 (PSEN1) | S319 | Uniprot |
| P49768 (PSEN1) | T320 | Uniprot |
| P50616 (TOB1) | S152 | Uniprot |
| P50616 (TOB1) | S154 | Uniprot |
| P50616 (TOB1) | S164 | Uniprot |
| P52945 (PDX1) | S61 | Uniprot |
| P52945 (PDX1) | S66 | Uniprot |
| P54259 (ATN1) | S739 | Uniprot |
| P55957 (BID) | T59 | Uniprot |
| P63104 (YWHAZ) | S184 | Uniprot |
| P68431 (HIST1H3J) | S29 | Uniprot |
| P84243 (H3F3B) | S29 | Uniprot |
| P98177 (FOXO4) | T451 | Uniprot |
| P98177 (FOXO4) | T455 | Uniprot |
| Q06481 (APLP2) | T736 | Uniprot |
| Q07817 (BCL2L1) | T47 | Uniprot |
| Q07817 (BCL2L1) | S62 | Uniprot |
| Q07817 (BCL2L1) | T115 | Uniprot |
| Q12968 (NFATC3) | S163 | Uniprot |
| Q12968-1 (NFATC3) | S165 | Uniprot |
| Q14653 (IRF3) | S173 | Uniprot |
| Q14934 (NFATC4) | S213 | Uniprot |
| Q14934 (NFATC4) | S217 | Uniprot |
| Q5JR12 (PPM1J) | S93 | Uniprot |
| Q8IW41 (MAPKAPK5) | T182 | Uniprot |
| Q8WYK2 (JDP2) | T148 | Uniprot |
| Q9BQQ3 (GORASP1) | S274 | Uniprot |
| Q9NYV6 (RRN3) | T200 | Uniprot |
| Q9UIG0 (BAZ1B) | S158 | Uniprot |
| Q9UPT6 (MAPK8IP3) | T265 | Uniprot |
| Q9UPT6 (MAPK8IP3) | T275 | Uniprot |
| Q9UPT6 (MAPK8IP3) | T286 | Uniprot |
| Q9UQF2 (MAPK8IP1) | T103 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (By similarity).
MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.
Cytoplasm. Nucleus.
Note: Colocalizes with POU5F1 in the nucleus.
Interacts with MECOM (By similarity). Interacts with DCLK2 (By similarity). Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway (By similarity). Interacts with NFATC4. Interacts with ATF7; the interaction does not phosphorylate ATF7 but acts as a docking site for ATF7-associated partners such as JUN. Interacts with BCL10. Interacts with CTNNB1 and GSK3B. Interacts with MAPKBP1. Interacts with POU5F1; phosphorylates POU5F1 at 'Ser-355'. Found in a complex with SH3RF1, RAC2, MAP3K7/TAK1, MAP2K7/MKK7, MAPK8IP1/JIP1 and MAPK8/JNK1 (By similarity).
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
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