TAF1B Antibody - #DF2402
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2402, RRID:AB_2839609.
折りたたみ／展開
MGC:9349; RAF1B; RAFI63; SL1; TAB1 B; TAF1B TATA box binding protein (TBP) associated factor, RNA polymerase I, B, 63kDa; TAFI63; TATA box binding protein associated factor 1B; TATA box binding protein associated factor RNA polymerase I subunit B;
免疫原
- Q53T94 TAF1B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLEEAEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTVEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIAKMAKTVKYDVQAVAIIVVVLKLLFLLDDSFEWSLSNLAEKHNEKNKKDKPWFDFRKWYQIMKKAFDEKKQKWEEARAKYLWKSEKPLYYSFVDKPVAYKKREMVVNLQKQFSTLVESTATAGKKSPSSFQFNWTEEDTDRTCFHGHSLQGVLKEKGQSLLTKNSLYWLSTQKFCRCYCTHVTTYEESNYSLSYQFILNLFSFLLRIKTSLLHEEVSLVEKKLFEKKYSVKRKKSRSKKVRRH
PTMs - Q53T94 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Acetylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| Y425 | Phosphorylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| Y434 | Phosphorylation | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| K440 | Acetylation | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot |
研究背景
Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.
Nucleus>Nucleolus.
Interacts with FLNA (via N-terminus) (By similarity). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3.
Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides (PubMed:21921198 and PubMed:21921199).
Belongs to the RRN7/TAF1B family.
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