Steroidogenic Factor 1 Antibody - #DF2406
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| 製品: | Steroidogenic Factor 1 Antibody |
| カタログ: | DF2406 |
| タンパク質の説明: | Rabbit polyclonal antibody to Steroidogenic Factor 1 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 55 kDa; 52kD(Calculated). |
| ユニプロット: | Q13285 |
| RRID: | AB_2839613 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2406, RRID:AB_2839613.
折りたたみ／展開
AD4BP; Adrenal 4 binding protein; Adrenal 4-binding protein; ELP; FTZ 1; FTZ1; FTZF 1; FTZF1; Fushi tarazu factor (Drosophila) homolog 1; Fushi tarazu factor homolog 1; hSF 1; NR5A1; Nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1; POF7; SF 1; SF-1; SF1; SPGF8; SRXY3; Steroid hormone receptor Ad4BP; Steroidogenic factor 1; Steroidogenic factor 1 nuclear receptor; STF 1; STF-1; STF1; STF1_HUMAN;
免疫原
High expressed in the adrenal cortex, the ovary, the testis, and the spleen (PubMed:9177385).
- Q13285 STF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPAPDYVLPPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPLAGYLYPAFPGRAIKSEYPEPYASPPQPGLPYGYPEPFSGGPNVPELILQLLQLEPDEDQVRARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQNCWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLALQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13285 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K34 | Acetylation | Uniprot | |
| K38 | Acetylation | Uniprot | |
| K72 | Acetylation | Uniprot | |
| K100 | Methylation | Uniprot | |
| K106 | Acetylation | PR:000007102 (EP300) | Uniprot |
| K109 | Acetylation | PR:000007102 (EP300) | Uniprot |
| K110 | Acetylation | PR:000007102 (EP300) | Uniprot |
| K119 | Sumoylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K194 | Sumoylation | Uniprot | |
| S203 | Phosphorylation | P28482 (MAPK1) , P50613 (CDK7) | Uniprot |
| S430 | Phosphorylation | Uniprot |
研究背景
Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Acetylation stimulates the transcriptional activity.
Sumoylation reduces CDK7-mediated phosphorylation on Ser-203.
Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity.
Nucleus.
High expressed in the adrenal cortex, the ovary, the testis, and the spleen.
Binds DNA as a monomer. Interacts with NR0B2 and PPARGC1A (By similarity). Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with NCOA2. Interacts with DGKQ and CDK7. Binds to and activated by HIPK3.
Belongs to the nuclear hormone receptor family. NR5 subfamily.
参考文献
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