Torc1/Crtc1 Antibody - #DF2415
製品: | Torc1/Crtc1 Antibody |
カタログ: | DF2415 |
タンパク質の説明: | Rabbit polyclonal antibody to Torc1/Crtc1 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse |
予測: | Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
分子量: | 78 kDa; 67kD(Calculated). |
ユニプロット: | Q6UUV9 |
RRID: | AB_2839621 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2415, RRID:AB_2839621.
折りたたみ/展開
KIAA0616; CREB regulated transcription coactivator 1; CREB-regulated transcription coactivator 1; CRTC1; CRTC1_HUMAN; FLJ14027; MECT 1; Mucoepidermoid carcinoma translocated 1; Mucoepidermoid carcinoma translocated protein 1; TORC-1; TORC1; Transducer of CREB protein 1; Transducer of regulated cAMP response element binding protein 1; Transducer of regulated cAMP response element-binding protein (CREB) 1; Transducer of regulated cAMP response element-binding protein 1; WAMTP1;
免疫原
Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
- Q6UUV9 CRTC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATSNNPRKFSEKIALHNQKQAEETAAFEEVMKDLSLTRAARLQLQKSQYLQLGPSRGQYYGGSLPNVNQIGSGTMDLPFQTPFQSSGLDTSRTTRHHGLVDRVYRERGRLGSPHRRPLSVDKHGRQADSCPYGTMYLSPPADTSWRRTNSDSALHQSTMTPTQPESFSSGSQDVHQKRVLLLTVPGMEETTSEADKNLSKQAWDTKKTGSRPKSCEVPGINIFPSADQENTTALIPATHNTGGSLPDLTNIHFPSPLPTPLDPEEPTFPALSSSSSTGNLAANLTHLGIGGAGQGMSTPGSSPQHRPAGVSPLSLSTEARRQQASPTLSPLSPITQAVAMDALSLEQQLPYAFFTQAGSQQPPPQPQPPPPPPPASQQPPPPPPPQAPVRLPPGGPLLPSASLTRGPQPPPLAVTVPSSLPQSPPENPGQPSMGIDIASAPALQQYRTSAGSPANQSPTSPVSNQGFSPGSSPQHTSTLGSVFGDAYYEQQMAARQANALSHQLEQFNMMENAISSSSLYSPGSTLNYSQAAMMGLTGSHGSLPDSQQLGYASHSGIPNIILTVTGESPPSLSKELTSSLAGVGDVSFDSDSQFPLDELKIDPLTLDGLHMLNDPDMVLADPATEDTFRMDRL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6UUV9 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K13 | Ubiquitination | Uniprot | |
K47 | Ubiquitination | Uniprot | |
S48 | Phosphorylation | Uniprot | |
Y50 | Phosphorylation | Uniprot | |
Y61 | Phosphorylation | Uniprot | |
S64 | Phosphorylation | Uniprot | |
R103 | Methylation | Uniprot | |
S113 | Phosphorylation | Uniprot | |
S120 | Phosphorylation | Uniprot | |
K123 | Acetylation | Uniprot | |
S130 | Phosphorylation | Uniprot | |
S139 | Phosphorylation | Uniprot | |
T149 | Phosphorylation | Uniprot | |
S151 | Phosphorylation | P57059 (SIK1) | Uniprot |
S153 | Phosphorylation | Uniprot | |
S158 | Phosphorylation | Uniprot | |
T161 | Phosphorylation | Uniprot | |
S167 | Phosphorylation | P57059 (SIK1) | Uniprot |
S172 | Phosphorylation | Uniprot | |
S215 | Phosphorylation | Uniprot | |
S312 | Phosphorylation | Uniprot | |
S330 | Phosphorylation | Uniprot | |
S333 | Phosphorylation | Uniprot | |
S424 | Phosphorylation | Uniprot |
研究背景
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.
(Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1 (By similarity).
Cytoplasm. Nucleus.
Note: Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).
Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Interacts with 14-3-3 proteins, including YWHAE/14-3-3 epsilon. Interacts with calmodulin-dependent catalytic subunit PPP3CA/calcineurin A.
(Microbial infection) Interacts with HTLV1 Tax.
Belongs to the TORC family.
研究領域
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
参考文献
Application: WB Species: mouse Sample: spinal cord
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