XRN1 Antibody - #DF2433
| 製品: | XRN1 Antibody |
| カタログ: | DF2433 |
| タンパク質の説明: | Rabbit polyclonal antibody to XRN1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse |
| 予測: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 194 kDa; 194kD(Calculated). |
| ユニプロット: | Q8IZH2 |
| RRID: | AB_2839639 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2433, RRID:AB_2839639.
折りたたみ／展開
5'-3' exoribonuclease 1; Dhm2; mXRN1; SEP1; Strand-exchange protein 1 homolog;
免疫原
Expressed in heart, brain, pancreas, spleen, testis, osteogenic sarcoma (OGS) biopsy and primary cell lines.
- Q8IZH2 XRN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREHFSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTDLNGKQQEWEAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPAIERCCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDELTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNWAKEVQGISEHYLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFDSRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDALIQNQHKYSIKYNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVKPQPAVHQHSSSSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVLPQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIENFLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMGTRMLKEILKIDGSNTVDHKNEIKQIANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVSTPVTELSRICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEAKEKAALFALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTGWDHYGSNYALGAANIMPSSSHLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFENKEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHHKSTPISSSRRKSRKLAVNFGVSKPSE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IZH2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K79 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K268 | Acetylation | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| Y384 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| K586 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| K673 | Ubiquitination | Uniprot | |
| K736 | Ubiquitination | Uniprot | |
| K747 | Ubiquitination | Uniprot | |
| T753 | Phosphorylation | Uniprot | |
| K758 | Ubiquitination | Uniprot | |
| K765 | Ubiquitination | Uniprot | |
| K772 | Ubiquitination | Uniprot | |
| Y797 | Phosphorylation | Uniprot | |
| T802 | Phosphorylation | Uniprot | |
| K805 | Ubiquitination | Uniprot | |
| K818 | Ubiquitination | Uniprot | |
| Y829 | Phosphorylation | Uniprot | |
| S844 | Phosphorylation | Uniprot | |
| K847 | Ubiquitination | Uniprot | |
| K910 | Ubiquitination | Uniprot | |
| R939 | Methylation | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| K971 | Ubiquitination | Uniprot | |
| Y990 | Phosphorylation | Uniprot | |
| K1035 | Ubiquitination | Uniprot | |
| K1130 | Ubiquitination | Uniprot | |
| S1173 | Phosphorylation | Uniprot | |
| S1176 | Phosphorylation | Uniprot | |
| K1228 | Ubiquitination | Uniprot | |
| K1245 | Ubiquitination | Uniprot | |
| Y1248 | Phosphorylation | Uniprot | |
| K1256 | Ubiquitination | Uniprot | |
| K1272 | Ubiquitination | Uniprot | |
| S1279 | Phosphorylation | Uniprot | |
| K1281 | Ubiquitination | Uniprot | |
| Y1282 | Phosphorylation | Uniprot | |
| S1299 | Phosphorylation | Uniprot | |
| K1301 | Ubiquitination | Uniprot | |
| S1306 | Phosphorylation | Uniprot | |
| K1308 | Ubiquitination | Uniprot | |
| S1348 | Phosphorylation | Uniprot | |
| K1364 | Acetylation | Uniprot | |
| K1364 | Ubiquitination | Uniprot | |
| K1374 | Ubiquitination | Uniprot | |
| K1378 | Ubiquitination | Uniprot | |
| Y1394 | Phosphorylation | Uniprot | |
| S1398 | Phosphorylation | Uniprot | |
| K1401 | Ubiquitination | Uniprot | |
| K1412 | Acetylation | Uniprot | |
| Y1419 | Phosphorylation | Uniprot | |
| S1451 | Phosphorylation | Uniprot | |
| K1621 | Ubiquitination | Uniprot | |
| T1629 | Phosphorylation | Uniprot | |
| T1633 | Phosphorylation | Uniprot | |
| S1638 | Phosphorylation | Uniprot | |
| S1645 | Phosphorylation | Uniprot | |
| S1653 | Phosphorylation | Uniprot | |
| S1692 | Phosphorylation | Uniprot |
研究背景
Major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'-processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). Plays a role in replication-dependent histone mRNA degradation. May act as a tumor suppressor protein in osteogenic sarcoma (OGS).
Cytoplasm.
Note: Discrete foci at the inner surface of the cell membrane.
Expressed in heart, brain, pancreas, spleen, testis, osteogenic sarcoma (OGS) biopsy and primary cell lines.
Found in a mRNP complex with UPF1, UPF2, UPF3B and XRN1. Associates with alpha and beta tubulins (By similarity). Interacts with DIS3L2. Interacts with ZC3HAV1 in an RNA-dependent manner. Interacts with ZFP36L1. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner. Interacts with YTHDC2 (via ANK repeats).
Belongs to the 5'-3' exonuclease family.
研究領域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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