FGFP1 Antibody - #DF2498

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製品: FGFP1 Antibody
カタログ: DF2498
タンパク質の説明: Rabbit polyclonal antibody to FGFP1
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human
予測: Bovine
分子量: 26-38kD; 26kD(Calculated).
ユニプロット: Q14512
RRID: AB_2839704

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Bovine(86%)
クローナリティ:
Polyclonal
特異性:
FGFP1 Antibody detects endogenous levels of total FGFP1.
RRID:
AB_2839704
引用形式: Affinity Biosciences Cat# DF2498, RRID:AB_2839704.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

17 kDa HBGF-binding protein; 17 kDa heparin binding growth factor binding protein; 17 kDa heparin-binding growth factor-binding protein; FGF binding protein 1; FGF BP; FGF BP1; FGF-binding protein 1; FGF-BP; FGF-BP1; FGFBP; FGFBP-1; Fgfbp1; FGFP1_HUMAN; Fibroblast growth factor binding protein 1 precursor; Fibroblast growth factor-binding protein 1; HBp17;

免疫原

免疫原:

A synthesized peptide derived from human FGFP1, corresponding to a region within C-terminal amino acids.

Uniprot:

Q14512(FGFP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
FGFBP1(9982)
発現特異性:
Q14512 FGFP1_HUMAN:

Expressed in the suprabasal region of the epidermis, in hair follicles, the basement membrane at the dermo-epidermal junction (occasionally extending into the basement membrane of dermal blood vessels), wounded skin and several invasive squamous cell carcinomas (at protein level). Expressed in normal and wounded skin and various squamous cell carcinomas.

タンパク質配列:
MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRWAATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCRKDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTALEFCGETWSSLCTFFLSIVQDTSC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
86
Pig
75
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Acts as a carrier protein that release fibroblast-binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth.

細胞の位置付け:

Secreted>Extracellular space. Cell membrane>Peripheral membrane protein.
Note: Extracellular and plasma membrane-associated. Colocalizes with HSPG2 in the pericellular environment of squamous cell carcinomas.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the suprabasal region of the epidermis, in hair follicles, the basement membrane at the dermo-epidermal junction (occasionally extending into the basement membrane of dermal blood vessels), wounded skin and several invasive squamous cell carcinomas (at protein level). Expressed in normal and wounded skin and various squamous cell carcinomas.

タンパク質ファミリー:

Belongs to the fibroblast growth factor-binding protein family.

参考文献

1). FGFBP1-mediated crosstalk between fibroblasts and pancreatic cancer cells via FGF22/FGFR2 promotes invasion and metastasis of pancreatic cancer. ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2021 (PubMed: 34117747) [IF=3.3]

Application: WB    Species: Human    Sample: PANC-1 and Mia PaCa-2 cells

Figure 1. FGFBP1 silencing in PCCs decreased the expression and secretion of FGF22 in human CAFs cocultured with PCCs (A) Efficiency of FGFBP1 knockdown in PANC-1 and Mia PaCa-2 cells evaluated by qRT-PCR. (B) Efficiency of FGFBP1 knockdown in PANC-1 and Mia PaCa-2 cells evaluated by western blot analysis. (C) Heat map of differentially expressed genes in mouse fibroblasts cocultured with FGFBP1-shRNA-transfected PANC-1 cells compared with mouse fibroblasts cocultured with scramble-transfected PANC-1 cells. (D) mRNA level of FGF22 in human CAFs cocultured with FGFBP1-shRNA-transfected PANC-1 or Mia PaCa-2 cells (CAF-P or CAF-M) compared with that in CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells. (E) Protein level of FGF22 in CAF-P or CAF-M compared with that in CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells are shown. (F) Secretion of FGF22 in culture medium of CAF-P or CAF-M compared with that in culture medium of CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells.

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